Restriction map for
pFN21AE6523
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstNI BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FokI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MseI MspA1I
MunI MvaI MvnI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AciI 242 GCGG CCGC(-3/-1)
AcyI 197 GGCGCC G[GA]^CG[CT]C
AluI 118 AGCT AG^CT
AlwI 206 GATCC GGATC(4/5)
AlwNI 82 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 110 GTGCTC G[AT]GC[AT]^C
AvaII 182 GGTCC G^G[AT]CC
BalI 258 TGGCCA TGG^CCA
BanI 197 GGCGCC G^G[CT][GA]CC
BbeI 197 GGCGCC GGCGC^C
BcnI 122 CCGGG CC^[GC]GG
BsaHI 197 GGCGCC G[GA]^CG[CT]C
BsiHKAI 110 GTGCTC G[AT]GC[AT]^C
BsiYI 215 CCTGTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 215 CCTGTGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrFI 194 GCCGGC [GA]^CCGG[CT]
BssKI 122 CCGGG ^CC[ACGT]GG
BstXI 261 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 205 AGATCC [GA]^GATC[CT]
CfoI 198 GCGC GCG^C
Cfr10I 194 GCCGGC [GA]^CCGG[CT]
DpnII 206 GATC ^GATC
DraII 181 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 258 TGGCCA [CT]^GGCC[GA]
EarI 99 CTCTTC CTCTTC(1/4)
EcoNI 215 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 181 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 261 CCATGG C^C[AT][AT]GG
FauI 242 GCGGG CCCGC(4/6)
HaeII 197 GGCGCC [GA]GCGC^[CT]
HhaI 198 GCGC GCG^C
Hin1I 197 GGCGCC G[GA]^CG[CT]C
HinP1I 198 GCGC G^CGC
HphI 52 TCACC GGTGA(8/7)
KasI 197 GGCGCC G^GCGCC
Ksp632I 99 CTCTTC CTCTTC(1/4)
MaeIII 218 GTGAC ^GT[ACGT]AC
MboI 206 GATC ^GATC
MboII 100 TCTTC GAAGA(8/7)
MflI 205 AGATCC [GA]^GATC[CT]
MluNI 258 TGGCCA TGG^CCA
MscI 258 TGGCCA TGG^CCA
MslI 53 CACCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 194 GCCGGC GCC^GGC
NarI 197 GGCGCC GG^CGCC
NciI 122 CCGGG CC^[GC]GG
NcoI 261 CCATGG C^CATGG
NdeII 206 GATC ^GATC
NgoMIV 194 GCCGGC G^CCGGC
NlaIII 262 CATG CATG^
PpuMI 181 AGGTCCC [GA]G^G[AT]CC[CT]
Sau3AI 206 GATC ^GATC
ScrFI 122 CCGGG CC^[ACGT]GG
SfoI 197 GGCGCC GGC^GCC
StyI 261 CCATGG C^C[AT][AT]GG
Tsp45I 218 GTGAC ^GT[GC]AC
XhoII 205 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BanII 72 GAGCCC G[GA]GC[CT]^C
246 GGGCTC
BsrI 41 CCAGT ACTGG(1/-1)
137 CCAGT
Cfr13I 133 GGCCC G^G[ACGT]CC
182 GGTCC
DdeI 115 CTAAG C^T[ACGT]AG
160 CTGAG
DsaI 255 CCGTGG C^C[GA][CT]GG
261 CCATGG
HaeIII 133 GGCC GG^CC
259 GGCC
HapII 122 CCGG C^CGG
195 CCGG
HpaII 122 CCGG C^CGG
195 CCGG
MspI 122 CCGG C^CGG
195 CCGG
Sau96I 133 GGCCC G^G[ACGT]CC
182 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BbvI BmyI BsaJI BsmFI Bsp1286I
Cac8I Fnu4HI ItaI MnlI MwoI
NlaIV TseI TspRI