Restriction map for
pFN21AE2185
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MaeI MamI MluI
MluNI MroI MscI MseI MslI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AlwI 18 GATCC GGATC(4/5)
AlwNI 368 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 637 GTGCTC G[AT]GC[AT]^C
AvaII 237 GGTCC G^G[AT]CC
BanI 376 GGCACC G^G[CT][GA]CC
BbvCI 128 CCTCAGC CCTCAGC(-5/-2)
BclI 315 TGATCA T^GATCA
BsaI 490 GAGACC GGTCTC(1/5)
BsgI 165 CTGCAC GTGCAG(16/14)
BsiHKAI 637 GTGCTC G[AT]GC[AT]^C
BsmI 417 GAATGC GAATGC(1/-1)
BsrI 439 ACTGG ACTGG(1/-1)
BstAPI 304 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 17 AGATCC [GA]^GATC[CT]
Cfr13I 237 GGTCC G^G[ACGT]CC
FbaI 315 TGATCA T^GATCA
HaeIII 295 GGCC GG^CC
HincII 553 GTTGAC GT[CT]^[GA]AC
HindII 553 GTTGAC GT[CT]^[GA]AC
HphI 747 TCACC GGTGA(8/7)
MaeII 356 ACGT A^CGT
MfeI 65 CAATTG C^AATTG
MflI 17 AGATCC [GA]^GATC[CT]
MunI 65 CAATTG C^AATTG
NspI 452 GCATGC [GA]CATG^[CT]
PflMI 800 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 744 GAGTC GAGTC(4/5)
Sau96I 237 GGTCC G^G[ACGT]CC
SfcI 751 CTACAG C^T[GA][CT]AG
SmlI 427 CTTGAG C^T[CT][GA]AG
SphI 452 GCATGC GCATG^C
Van91I 800 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 17 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbvI 164 GCTGC GCAGC(8/12)
756 GCTGC
BmyI 637 GTGCTC G[AGT]GC[ACT]^C
809 GGGCAC
Bsp1286I 637 GTGCTC G[AGT]GC[ACT]^C
809 GGGCAC
BsrDI 690 CATTGC GCAATG(2/0)
332 GCAATG
BssKI 9 CCTGG ^CC[ACGT]GG
558 CCAGG
BstNI 9 CCTGG CC^[AT]GG
558 CCAGG
EarI 22 CTCTTC CTCTTC(1/4)
786 CTCTTC
EcoRII 9 CCTGG ^CC[AT]GG
558 CCAGG
EcoRV 196 GATATC GAT^ATC
709 GATATC
HapII 297 CCGG C^CGG
457 CCGG
HpaII 297 CCGG C^CGG
457 CCGG
Ksp632I 22 CTCTTC CTCTTC(1/4)
786 CTCTTC
MboII 23 TCTTC GAAGA(8/7)
787 TCTTC
MspI 297 CCGG C^CGG
457 CCGG
MvaI 9 CCTGG CC^[AT]GG
558 CCAGG
ScrFI 9 CCTGG CC^[ACGT]GG
558 CCAGG
TfiI 155 GAATC G^A[AT]TC
724 GAATC
TseI 164 GCTGC G^C[AT]GC
756 GCTGC
Tsp509I 36 AATT ^AATT
66 AATT
TspRI 305 CAGTG CA[GC]TG[ACGT][ACGT]^
533 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BpmI BsaJI BsiYI
BslI BsmAI BstF5I Cac8I DdeI
DpnII Fnu4HI FokI HinfI ItaI
MaeIII MboI MnlI MspA1I MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
SfaNI Tsp45I