Restriction map for
pFN21AE1961
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI BamHI BbeI BbrPI
BbsI BcgI BclI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HaeII Hin1I HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1328 GGTACC G^GTACC
AccI 931 GTCTAC GT^[AC][GT]AC
AflII 1447 CTTAAG C^TTAAG
AlwNI 1273 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1341 GGGCCC GGGCC^C
Asp700I 173 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1328 GGTACC G^GTACC
AviII 1226 TGCGCA TGC^GCA
AvrII 1168 CCTAGG C^CTAGG
BbvCI 1129 GCTGAGG CCTCAGC(-5/-2)
BfrI 1447 CTTAAG C^TTAAG
BglI 1177 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1168 CCTAGG C^CTAGG
BsaI 220 GAGACC GGTCTC(1/5)
BsaWI 638 ACCGGA [AT]^CCGG[AT]
BsmBI 1120 CGTCTC CGTCTC(1/5)
BsmI 469 GCATTC GAATGC(1/-1)
BsrBI 765 CCGCTC CCGCTC(-3/-3)
BsrDI 273 CATTGC GCAATG(2/0)
BstBI 48 TTCGAA TT^CGAA
BstXI 908 CCAAGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FspI 1226 TGCGCA TGC^GCA
HgaI 1119 GCGTC GACGC(5/10)
KpnI 1328 GGTACC GGTAC^C
MseI 1448 TTAA T^TAA
NdeI 913 CATATG CA^TATG
NspI 533 ACATGC [GA]CATG^[CT]
NspV 48 TTCGAA TT^CGAA
PpuMI 496 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 89 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 724 GAGCTC GAGCT^C
SfiI 1176 GGCCGCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 48 TTCGAA TT^CGAA
SmaI 974 CCCGGG CCC^GGG
TaqI 49 TCGA T^CGA
Tru9I 1448 TTAA T^TAA
TthHB8I 49 TCGA T^CGA
XcmI 710 CCATCGTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 974 CCCGGG C^CCGGG
XmaI 974 CCCGGG C^CCGGG
XmnI 173 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 1221 GTAC GT^AC
1329 GTAC
AlwI 405 GATCC GGATC(4/5)
281 GGATC
AspHI 250 GAGCAC G[AT]GC[AT]^C
724 GAGCTC
AvaI 632 CTCGGG C^[CT]CG[GA]G
974 CCCGGG
BanI 241 GGCACC G^G[CT][GA]CC
1328 GGTACC
BciVI 3 GGATAC GTATCC(6/5)
812 GTATCC
BsiHKAI 250 GAGCAC G[AT]GC[AT]^C
724 GAGCTC
BsoBI 632 CTCGGG C^[CT]CG[GA]G
974 CCCGGG
BspMI 1223 ACCTGC ACCTGC(4/8)
756 GCAGGT
CfoI 1002 GCGC GCG^C
1227 GCGC
DpnII 282 GATC ^GATC
405 GATC
DraII 496 GGGACCT [GA]G^G[ACGT]CC[CT]
1341 GGGCCCC
EcoO109I 496 GGGACCT [GA]G^G[ACGT]CC[CT]
1341 GGGCCCC
FauI 785 CCCGC CCCGC(4/6)
1339 GCGGG
HhaI 1002 GCGC GCG^C
1227 GCGC
HinP1I 1002 GCGC G^CGC
1227 GCGC
MboI 282 GATC ^GATC
405 GATC
NcoI 464 CCATGG C^CATGG
1199 CCATGG
NdeII 282 GATC ^GATC
405 GATC
PflMI 699 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1251 CCACAGCATGG
PvuII 733 CAGCTG CAG^CTG
992 CAGCTG
RsaI 1221 GTAC GT^AC
1329 GTAC
Sau3AI 282 GATC ^GATC
405 GATC
SmlI 1011 CTCAAG C^T[CT][GA]AG
1447 CTTAAG
StuI 1100 AGGCCT AGG^CCT
1313 AGGCCT
TfiI 10 GAATC G^A[AT]TC
178 GATTC
Van91I 699 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1251 CCACAGCATGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
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AciI AcsI AluI ApoI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DsaI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PleI PstI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI