Restriction map for
pFN21AE3879
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MaeII MluI MluNI
MroI MscI MspA1I MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflII 1531 CTTAAG C^TTAAG
AspHI 1585 GAGCTC G[AT]GC[AT]^C
AvaII 2859 GGACC G^G[AT]CC
BanII 1585 GAGCTC G[GA]GC[CT]^C
BclI 2481 TGATCA T^GATCA
BfrI 1531 CTTAAG C^TTAAG
BmyI 1585 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 1585 GAGCTC G[AT]GC[AT]^C
BsiYI 167 CCAAAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 167 CCAAAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 1585 GAGCTC G[AGT]GC[ACT]^C
BsrDI 2549 CATTGC GCAATG(2/0)
BsrI 708 ACTGG ACTGG(1/-1)
BstF5I 1670 GGATG GGATG(2/0)
DraII 2792 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1226 CCATGG C^C[GA][CT]GG
EcoO109I 2792 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 2016 GATATC GAT^ATC
EcoT14I 1226 CCATGG C^C[AT][AT]GG
EcoT22I 715 ATGCAT ATGCA^T
FauI 2853 GCGGG CCCGC(4/6)
FbaI 2481 TGATCA T^GATCA
FokI 1670 GGATG GGATG(9/13)
HpaI 1452 GTTAAC GTT^AAC
HphI 66 GGTGA GGTGA(8/7)
NcoI 1226 CCATGG C^CATGG
NlaIV 2793 GGCCCC GG[ACGT]^[ACGT]CC
NsiI 715 ATGCAT ATGCA^T
SacI 1585 GAGCTC GAGCT^C
SapI 1741 GAAGAGC GCTCTTC(1/4)
ScaI 1006 AGTACT AGT^ACT
StuI 1868 AGGCCT AGG^CCT
StyI 1226 CCATGG C^C[AT][AT]GG
TaqI 2463 TCGA T^CGA
Tsp45I 254 GTCAC ^GT[GC]AC
TthHB8I 2463 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 64 GCGG CCGC(-3/-1)
2853 GCGG
AseI 1642 ATTAAT AT^TAAT
2668 ATTAAT
AsnI 1642 ATTAAT AT^TAAT
2668 ATTAAT
Asp700I 1687 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1846 GAAGAACTTC
BbsI 2571 GAAGAC GAAGAC(2/6)
1406 GTCTTC
BglII 574 AGATCT A^GATCT
1500 AGATCT
BpuAI 2571 GAAGAC GAAGAC(2/6)
1406 GTCTTC
BsaBI 187 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
816 GATCCAAATC
BspMI 234 GCAGGT ACCTGC(4/8)
798 GCAGGT
Cac8I 690 GCAAGC GC[ACGT]^[ACGT]GC
1898 GCAAGC
Cfr13I 2793 GGCCC G^G[ACGT]CC
2859 GGACC
EcoRI 291 GAATTC G^AATTC
699 GAATTC
HincII 1452 GTTAAC GT[CT]^[GA]AC
2108 GTCAAC
HindII 1452 GTTAAC GT[CT]^[GA]AC
2108 GTCAAC
MamI 187 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
816 GATCCAAATC
MfeI 938 CAATTG C^AATTG
1210 CAATTG
MslI 2155 CACCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2767 CATCTTTGTG
MunI 938 CAATTG C^AATTG
1210 CAATTG
PleI 277 GACTC GAGTC(4/5)
2185 GACTC
PshBI 1642 ATTAAT AT^TAAT
2668 ATTAAT
PstI 578 CTGCAG CTGCA^G
1152 CTGCAG
Sau96I 2793 GGCCC G^G[ACGT]CC
2859 GGACC
SfaNI 951 GCATC GCATC(5/9)
2239 GCATC
XmnI 1687 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1846 GAAGAACTTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
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AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BfaI BsaJI BsmAI
BssKI BstNI BstYI DdeI DpnII
EarI EcoRII Fnu4HI HaeIII HindIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI ScrFI SfcI SmlI TfiI
Tru9I TseI Tsp509I TspRI XhoII