Restriction map for
pFN21AE4183
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BbrPI BbvCI BcgI BclI
BfaI BfrI BlnI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MaeI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfcI
SfiI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 842 ACGTGT A^C[GA][CT]GT
AviII 1700 TGCGCA TGC^GCA
BbeI 850 GGCGCC GGCGC^C
BciVI 1105 GGATAC GTATCC(6/5)
BglI 1613 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1142 AGATCT A^GATCT
BsaAI 559 TACGTG [CT]AC^GT[GA]
BsaBI 1725 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 607 GAGACG CGTCTC(1/5)
BsmI 1314 GAATGC GAATGC(1/-1)
BsrBI 433 GAGCGG CCGCTC(-3/-3)
BsrFI 60 GCCGGT [GA]^CCGG[CT]
BssSI 1522 CACGAG CACGAG(-5/-1)
BstBI 1311 TTCGAA TT^CGAA
Cfr10I 60 GCCGGT [GA]^CCGG[CT]
DraIII 690 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1347 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1316 ATGCAT ATGCA^T
FspI 1700 TGCGCA TGC^GCA
KasI 850 GGCGCC G^GCGCC
MamI 1725 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1778 TTAA T^TAA
MspA1I 273 CCGCTG C[AC]G^C[GT]G
NarI 850 GGCGCC GG^CGCC
NcoI 986 CCATGG C^CATGG
NsiI 1316 ATGCAT ATGCA^T
NspI 1706 GCATGC [GA]CATG^[CT]
NspV 1311 TTCGAA TT^CGAA
PflMI 47 CCATCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 257 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1714 GAGCTC GAGCT^C
ScaI 242 AGTACT AGT^ACT
SfoI 850 GGCGCC GGC^GCC
SfuI 1311 TTCGAA TT^CGAA
SmlI 1164 CTTGAG C^T[CT][GA]AG
SphI 1706 GCATGC GCATG^C
StuI 1620 AGGCCT AGG^CCT
TfiI 1236 GAATC G^A[AT]TC
Tru9I 1778 TTAA T^TAA
Van91I 47 CCATCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 850 GGCGCC G[GA]^CG[CT]C
1171 GGCGTC
AlwNI 913 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1193 CAGTCCCTG
Asp700I 210 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1140 GAAGATCTTC
BamHI 923 GGATCC G^GATCC
1286 GGATCC
BlpI 486 GCTCAGC GC^T[ACGT]AGC
1716 GCTCAGC
Bpu1102I 486 GCTCAGC GC^T[ACGT]AGC
1716 GCTCAGC
BsaHI 850 GGCGCC G[GA]^CG[CT]C
1171 GGCGTC
BsaI 1803 GAGACC GGTCTC(1/5)
1953 GGTCTC
BsrDI 1182 CATTGC GCAATG(2/0)
1940 GCAATG
CelII 486 GCTCAGC GC^T[ACGT]AGC
1716 GCTCAGC
DsaI 713 CCGTGG C^C[GA][CT]GG
986 CCATGG
EagI 5 CGGCCG C^GGCCG
92 CGGCCG
EclXI 5 CGGCCG C^GGCCG
92 CGGCCG
Eco52I 5 CGGCCG C^GGCCG
92 CGGCCG
EcoT14I 236 CCAAGG C^C[AT][AT]GG
986 CCATGG
Hin1I 850 GGCGCC G[GA]^CG[CT]C
1171 GGCGTC
PleI 454 GACTC GAGTC(4/5)
472 GAGTC
SapI 942 GAAGAGC GCTCTTC(1/4)
732 GCTCTTC
SmaI 35 CCCGGG CCC^GGG
630 CCCGGG
StyI 236 CCAAGG C^C[AT][AT]GG
986 CCATGG
Tsp509I 1675 AATT ^AATT
1780 AATT
XcmI 1091 CCATGACCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1283 CCAGGATCCGTGTGG
XmaCI 35 CCCGGG C^CCGGG
630 CCCGGG
XmaI 35 CCCGGG C^CCGGG
630 CCCGGG
XmnI 210 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1140 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Aor51HI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco47III EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I XhoII