Restriction map for
pFN21AE4175
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaII AvrII BbvCI BcgI
BciVI BfrI BglII BlnI BsiWI
BsmBI Bsp1407I BspDI BspHI BsrDI
BsrGI BssHII Bst1107I BstXI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoNI EcoRI EcoRV EcoT22I
HindIII HpaI KpnI MfeI MunI
NdeI NheI NotI NruI NsiI
PacI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tsp509I XbaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 1811 TCCGGA T^CCGGA
BamHI 651 GGATCC G^GATCC
BbeI 595 GGCGCC GGCGC^C
BbrPI 1483 CACGTG CAC^GTG
BbsI 304 GTCTTC GAAGAC(2/6)
BclI 2 TGATCA T^GATCA
BfaI 674 CTAG C^TAG
BpuAI 304 GTCTTC GAAGAC(2/6)
BsaBI 111 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1811 TCCGGA [AT]^CCGG[AT]
BseAI 1811 TCCGGA T^CCGGA
BsgI 343 CTGCAC GTGCAG(16/14)
BsmI 1851 GCATTC GAATGC(1/-1)
BspEI 1811 TCCGGA T^CCGGA
BspMI 935 ACCTGC ACCTGC(4/8)
BssSI 574 CACGAG CACGAG(-5/-1)
BstBI 376 TTCGAA TT^CGAA
BstEII 1293 GGTCACC G^GT[ACGT]ACC
BstPI 1293 GGTCACC G^GT[ACGT]ACC
EagI 1492 CGGCCG C^GGCCG
EclXI 1492 CGGCCG C^GGCCG
Eco52I 1492 CGGCCG C^GGCCG
EcoO65I 1293 GGTCACC G^GT[ACGT]ACC
FbaI 2 TGATCA T^GATCA
FseI 214 GGCCGGCC GGCCGG^CC
HincII 1414 GTCAAC GT[CT]^[GA]AC
HindII 1414 GTCAAC GT[CT]^[GA]AC
KasI 595 GGCGCC G^GCGCC
MaeI 674 CTAG C^TAG
MamI 111 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1101 ACGCGT A^CGCGT
MroI 1811 TCCGGA T^CCGGA
MseI 752 TTAA T^TAA
NarI 595 GGCGCC GG^CGCC
NspV 376 TTCGAA TT^CGAA
PaeR7I 1736 CTCGAG C^TCGAG
PmaCI 1483 CACGTG CAC^GTG
PmlI 1483 CACGTG CAC^GTG
PstI 556 CTGCAG CTGCA^G
SacI 385 GAGCTC GAGCT^C
SfoI 595 GGCGCC GGC^GCC
SfuI 376 TTCGAA TT^CGAA
SmlI 1736 CTCGAG C^T[CT][GA]AG
StuI 746 AGGCCT AGG^CCT
Tru9I 752 TTAA T^TAA
XhoI 1736 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 805 GTGCAC G^TGCAC
1367 GTGCAC
AlwNI 559 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
1456 CAGAAGCTG
AocI 1119 CCTGAGG CC^T[ACGT]AGG
1858 CCTGAGG
ApaLI 805 GTGCAC G^TGCAC
1367 GTGCAC
AspI 922 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1335 GACGTGGTC
BglI 187 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
460 GCCGAGGAGGC
BsaAI 313 TACGTG [CT]AC^GT[GA]
1483 CACGTG
BsiEI 759 CGACCG CG[GA][CT]^CG
1492 CGGCCG
BspLU11I 67 ACATGT A^CATGT
1322 ACATGT
BstYI 651 GGATCC [GA]^GATC[CT]
776 AGATCC
Bsu36I 1119 CCTGAGG CC^T[ACGT]AGG
1858 CCTGAGG
DraIII 1087 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1361 CACTGTGTG
Eco81I 1119 CCTGAGG CC^T[ACGT]AGG
1858 CCTGAGG
MflI 651 GGATCC [GA]^GATC[CT]
776 AGATCC
NcoI 591 CCATGG C^CATGG
1445 CCATGG
PflFI 922 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1335 GACGTGGTC
PvuII 1501 CAGCTG CAG^CTG
1757 CAGCTG
SapI 372 GCTCTTC GCTCTTC(1/4)
642 GCTCTTC
SfcI 556 CTGCAG C^T[GA][CT]AG
1557 CTACAG
SphI 1346 GCATGC GCATG^C
1867 GCATGC
Tth111I 922 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1335 GACGTGGTC
XcmI 722 CCACTTTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1298 CCATGACGACACTGG
XhoII 651 GGATCC [GA]^GATC[CT]
776 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AspHI AvaI
AviII BalI BanI BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XmaCI XmaI