Restriction map for
pFN21AB8821
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BamHI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MboI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NdeII
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AfaI 456 GTAC GT^AC
AflIII 1007 ACACGT A^C[GA][CT]GT
AgeI 37 ACCGGT A^CCGGT
ApaI 762 GGGCCC GGGCC^C
BbrPI 1008 CACGTG CAC^GTG
BbsI 874 GTCTTC GAAGAC(2/6)
BbvCI 231 CCTCAGC CCTCAGC(-5/-2)
BpuAI 874 GTCTTC GAAGAC(2/6)
BsaAI 1008 CACGTG [CT]AC^GT[GA]
BsaWI 37 ACCGGT [AT]^CCGG[AT]
BsiEI 1073 CGGCCG CG[GA][CT]^CG
BsmAI 839 GTCTC GTCTC(1/5)
BsmBI 838 CGTCTC CGTCTC(1/5)
BsrBI 1032 CCGCTC CCGCTC(-3/-3)
BstAPI 702 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 762 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 229 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1073 CGGCCG C^GGCCG
EarI 67 GAAGAG CTCTTC(1/4)
EclXI 1073 CGGCCG C^GGCCG
Eco52I 1073 CGGCCG C^GGCCG
EcoO109I 762 GGGCCCC [GA]G^G[ACGT]CC[CT]
Ksp632I 67 GAAGAG CTCTTC(1/4)
MaeII 1009 ACGT A^CGT
MaeIII 102 GTCAC ^GT[ACGT]AC
NaeI 86 GCCGGC GCC^GGC
NgoMIV 86 GCCGGC G^CCGGC
NotI 1072 GCGGCCGC GC^GGCCGC
NspI 479 GCATGC [GA]CATG^[CT]
PinAI 37 ACCGGT A^CCGGT
PmaCI 1008 CACGTG CAC^GTG
PmlI 1008 CACGTG CAC^GTG
PstI 184 CTGCAG CTGCA^G
PvuII 960 CAGCTG CAG^CTG
RsaI 456 GTAC GT^AC
SfcI 184 CTGCAG C^T[GA][CT]AG
SgrAI 36 CACCGGTG C[GA]^CCGG[CT]G
SphI 479 GCATGC GCATG^C
TfiI 566 GAATC G^A[AT]TC
Tsp45I 102 GTCAC ^GT[GC]AC
XcmI 887 CCACGCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 268 GTGCTC G[AT]GC[AT]^C
682 GTGCTC
BglI 589 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
764 GCCCCGGTGGC
BsiHKAI 268 GTGCTC G[AT]GC[AT]^C
682 GTGCTC
BspMI 416 ACCTGC ACCTGC(4/8)
695 ACCTGC
BstXI 143 CCAACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCACTGGTGTGG
HphI 906 GGTGA GGTGA(8/7)
425 TCACC
MboII 67 GAAGA GAAGA(8/7)
875 TCTTC
NlaIII 150 CATG CATG^
480 CATG
PleI 368 GACTC GAGTC(4/5)
73 GAGTC
SmaI 428 CCCGGG CCC^GGG
741 CCCGGG
XmaCI 428 CCCGGG C^CCGGG
741 CCCGGG
XmaI 428 CCCGGG C^CCGGG
741 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI BalI BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
KasI KspI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIV SacII
Sau96I ScrFI SfaNI SfoI TseI
TspRI