Restriction map for
pFN21AE2372
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BlpI BmyI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I FauI FbaI FseI FspI
HaeIII HapII HincII HindII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI TspRI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1834 CGCG CG^CG
AcyI 597 GGCGTC G[GA]^CG[CT]C
AlwNI 634 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1920 AGCGCT AGC^GCT
BamHI 156 GGATCC G^GATCC
BbrPI 1413 CACGTG CAC^GTG
BglI 661 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 264 AGATCT A^GATCT
BpmI 332 CTCCAG CTGGAG(16/14)
BsaAI 1413 CACGTG [CT]AC^GT[GA]
BsaHI 597 GGCGTC G[GA]^CG[CT]C
BsgI 1490 CTGCAC GTGCAG(16/14)
BsrDI 276 GCAATG GCAATG(2/0)
BssSI 931 CACGAG CACGAG(-5/-1)
BstBI 1307 TTCGAA TT^CGAA
BstF5I 738 CATCC GGATG(2/0)
BstUI 1834 CGCG CG^CG
CfoI 1921 GCGC GCG^C
DraII 1470 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 1920 AGCGCT AGC^GCT
EcoNI 1657 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1470 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 906 GAATTC G^AATTC
EcoRV 1632 GATATC GAT^ATC
EcoT14I 307 CCAAGG C^C[AT][AT]GG
EcoT22I 1706 ATGCAT ATGCA^T
FokI 738 CATCC GGATG(9/13)
HaeII 1920 AGCGCT [GA]GCGC^[CT]
HhaI 1921 GCGC GCG^C
Hin1I 597 GGCGTC G[GA]^CG[CT]C
HinP1I 1921 GCGC G^CGC
HindIII 1102 AAGCTT A^AGCTT
MspA1I 868 CAGCGG C[AC]G^C[GT]G
MvnI 1834 CGCG CG^CG
NsiI 1706 ATGCAT ATGCA^T
NspI 291 ACATGC [GA]CATG^[CT]
NspV 1307 TTCGAA TT^CGAA
PflMI 1567 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1413 CACGTG CAC^GTG
PmlI 1413 CACGTG CAC^GTG
PpuMI 1470 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1130 CTGCAG CTGCA^G
SfuI 1307 TTCGAA TT^CGAA
SmlI 284 CTCAAG C^T[CT][GA]AG
SpeI 24 ACTAGT A^CTAGT
StyI 307 CCAAGG C^C[AT][AT]GG
TfiI 1792 GAATC G^A[AT]TC
Tsp45I 600 GTCAC ^GT[GC]AC
Van91I 1567 CCAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1869 CCATCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 232 GTAGAC GT^[AC][GT]AC
1553 GTAGAC
AfaI 281 GTAC GT^AC
1090 GTAC
Asp700I 193 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1614 GAATACCTTC
AvaII 1002 GGACC G^G[AT]CC
1471 GGACC
BbsI 224 GTCTTC GAAGAC(2/6)
1935 GTCTTC
BpuAI 224 GTCTTC GAAGAC(2/6)
1935 GTCTTC
BsmFI 1470 GGGAC GGGAC(10/14)
28 GTCCC
BspMI 357 ACCTGC ACCTGC(4/8)
36 GCAGGT
BsrI 531 ACTGG ACTGG(1/-1)
112 CCAGT
Cfr13I 1002 GGACC G^G[ACGT]CC
1471 GGACC
DraI 207 TTTAAA TTT^AAA
366 TTTAAA
HgaI 598 GCGTC GACGC(5/10)
1933 GCGTC
MaeII 947 ACGT A^CGT
1414 ACGT
NlaIII 292 CATG CATG^
694 CATG
NlaIV 156 GGATCC GG[ACGT]^[ACGT]CC
1470 GGGACC
PleI 814 GACTC GAGTC(4/5)
1838 GACTC
RsaI 281 GTAC GT^AC
1090 GTAC
Sau96I 1002 GGACC G^G[ACGT]CC
1471 GGACC
TaqI 1308 TCGA T^CGA
1630 TCGA
TthHB8I 1308 TCGA T^CGA
1630 TCGA
XmnI 193 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1614 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmAI BsmI BssKI BstNI
BstYI Cac8I DdeI DpnII EarI
EcoRII Fnu4HI HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII Sau3AI ScrFI SfaNI SfcI
Tru9I TseI Tsp509I XhoII