Restriction map for
pFN21AE6661
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FokI
FseI FspI HapII HgaI Hin1I
HindIII HpaI HpaII KasI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TseI Tsp45I
Tth111I TthHB8I Van91I XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 487 GGTACC G^GTACC
AfaI 488 GTAC GT^AC
AlwI 646 GATCC GGATC(4/5)
ApaI 41 GGGCCC GGGCC^C
Asp718I 487 GGTACC G^GTACC
BanII 41 GGGCCC G[GA]GC[CT]^C
BciVI 137 GTATCC GTATCC(6/5)
BclI 374 TGATCA T^GATCA
BglI 729 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 121 GAGGAG GAGGAG(10/8)
BspHI 129 TCATGA T^CATGA
BsrDI 378 CATTGC GCAATG(2/0)
BssKI 33 CCTGG ^CC[ACGT]GG
BssSI 718 CACGAG CACGAG(-5/-1)
BstNI 33 CCTGG CC^[AT]GG
DdeI 456 CTTAG C^T[ACGT]AG
EaeI 20 CGGCCA [CT]^GGCC[GA]
EcoRII 33 CCTGG ^CC[AT]GG
FbaI 374 TGATCA T^GATCA
HaeII 27 GGCGCT [GA]GCGC^[CT]
HphI 385 TCACC GGTGA(8/7)
KpnI 487 GGTACC GGTAC^C
MaeIII 554 GTAAC ^GT[ACGT]AC
MvaI 33 CCTGG CC^[AT]GG
PleI 636 GACTC GAGTC(4/5)
PstI 58 CTGCAG CTGCA^G
RcaI 129 TCATGA T^CATGA
RsaI 488 GTAC GT^AC
SapI 685 GCTCTTC GCTCTTC(1/4)
ScrFI 33 CCTGG CC^[ACGT]GG
SfaNI 585 GATGC GCATC(5/9)
SfcI 58 CTGCAG C^T[GA][CT]AG
XbaI 498 TCTAGA T^CTAGA
XcmI 23 CCAAGGCGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BfaI 317 CTAG C^TAG
499 CTAG
BsgI 52 CTGCAC GTGCAG(16/14)
323 GTGCAG
BsmI 211 GAATGC GAATGC(1/-1)
698 GCATTC
BspMI 56 ACCTGC ACCTGC(4/8)
71 ACCTGC
BsrI 80 ACTGG ACTGG(1/-1)
437 ACTGG
EarI 303 CTCTTC CTCTTC(1/4)
686 CTCTTC
FauI 44 CCCGC CCCGC(4/6)
39 GCGGG
HincII 328 GTTGAC GT[CT]^[GA]AC
633 GTTGAC
HindII 328 GTTGAC GT[CT]^[GA]AC
633 GTTGAC
Ksp632I 303 CTCTTC CTCTTC(1/4)
686 CTCTTC
MaeI 317 CTAG C^TAG
499 CTAG
MseI 407 TTAA T^TAA
563 TTAA
TfiI 295 GAATC G^A[AT]TC
367 GATTC
Tru9I 407 TTAA T^TAA
563 TTAA
TspRI 216 CACTG CA[GC]TG[ACGT][ACGT]^
529 CAGTG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI Asp700I BanI
BmyI BsaJI BsiYI BslI Bsp1286I
BstUI Cac8I CfoI Cfr13I DpnII
EcoT14I Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI MboI MboII MnlI
MspA1I MvnI MwoI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I StyI
Tsp509I XmnI