Restriction map for
pFN21AE9566
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AviII AvrII BalI BamHI
BanII BbeI BbrPI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HinfI HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Van91I XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 156 CGCG CG^CG
AcsI 178 AAATTT [GA]^AATT[CT]
AcyI 953 GGCGTC G[GA]^CG[CT]C
AlwI 623 GGATC GGATC(4/5)
ApoI 178 AAATTT [GA]^AATT[CT]
AspI 573 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 759 CCCGGG C^[CT]CG[GA]G
BanI 904 GGCACC G^G[CT][GA]CC
BbsI 714 GAAGAC GAAGAC(2/6)
BbvCI 981 CCTCAGC CCTCAGC(-5/-2)
BclI 164 TGATCA T^GATCA
BpuAI 714 GAAGAC GAAGAC(2/6)
BsaAI 628 TACGTG [CT]AC^GT[GA]
BsaHI 953 GGCGTC G[GA]^CG[CT]C
BseRI 778 CTCCTC GAGGAG(10/8)
BsmAI 660 GTCTC GTCTC(1/5)
BsoBI 759 CCCGGG C^[CT]CG[GA]G
BspHI 392 TCATGA T^CATGA
BspMI 617 ACCTGC ACCTGC(4/8)
BstUI 156 CGCG CG^CG
BstYI 623 GGATCT [GA]^GATC[CT]
CfoI 844 GCGC GCG^C
DsaI 371 CCGTGG C^C[GA][CT]GG
EarI 528 CTCTTC CTCTTC(1/4)
EcoT14I 1029 CCAAGG C^C[AT][AT]GG
FauI 402 GCGGG CCCGC(4/6)
FbaI 164 TGATCA T^GATCA
HaeII 843 GGCGCT [GA]GCGC^[CT]
HgaI 954 GCGTC GACGC(5/10)
HhaI 844 GCGC GCG^C
Hin1I 953 GGCGTC G[GA]^CG[CT]C
HinP1I 844 GCGC G^CGC
HincII 295 GTCAAC GT[CT]^[GA]AC
HindII 295 GTCAAC GT[CT]^[GA]AC
HindIII 85 AAGCTT A^AGCTT
Ksp632I 528 CTCTTC CTCTTC(1/4)
MflI 623 GGATCT [GA]^GATC[CT]
MseI 263 TTAA T^TAA
MspA1I 300 CCGCTG C[AC]G^C[GT]G
MvnI 156 CGCG CG^CG
PflFI 573 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 795 CTGCAG CTGCA^G
RcaI 392 TCATGA T^CATGA
SapI 527 GCTCTTC GCTCTTC(1/4)
SfcI 795 CTGCAG C^T[GA][CT]AG
SmaI 759 CCCGGG CCC^GGG
StyI 1029 CCAAGG C^C[AT][AT]GG
Tru9I 263 TTAA T^TAA
Tth111I 573 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 623 GGATCT [GA]^GATC[CT]
XmaCI 759 CCCGGG C^CCGGG
XmaI 759 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BcnI 759 CCCGG CC^[GC]GG
760 CCGGG
BsgI 811 GTGCAG GTGCAG(16/14)
1034 GTGCAG
BspLU11I 383 ACATGT A^CATGT
896 ACATGT
BstF5I 966 CATCC GGATG(2/0)
398 GGATG
BstNI 534 CCTGG CC^[AT]GG
765 CCTGG
EcoRII 534 CCTGG ^CC[AT]GG
765 CCTGG
FokI 966 CATCC GGATG(9/13)
398 GGATG
HapII 690 CCGG C^CGG
760 CCGG
HpaII 690 CCGG C^CGG
760 CCGG
MspI 690 CCGG C^CGG
760 CCGG
MvaI 534 CCTGG CC^[AT]GG
765 CCTGG
NciI 759 CCCGG CC^[GC]GG
760 CCGGG
SfaNI 399 GATGC GCATC(5/9)
965 GCATC
TaqI 65 TCGA T^CGA
782 TCGA
TthHB8I 65 TCGA T^CGA
782 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AflIII AluI AvaII
BbvI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstXI Cac8I Cfr13I
DdeI DpnII Fnu4HI HaeIII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI TseI Tsp45I Tsp509I TspRI