Restriction map for
pFN21AE2035
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 480 ACATGT A^C[GA][CT]GT
AlwNI 1759 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1477 CCTGAGG CC^T[ACGT]AGG
BalI 93 TGGCCA TGG^CCA
BanI 1031 GGCACC G^G[CT][GA]CC
BfaI 604 CTAG C^TAG
BpmI 1372 CTGGAG CTGGAG(16/14)
BsgI 300 CTGCAC GTGCAG(16/14)
BsmFI 314 GTCCC GGGAC(10/14)
BspLU11I 480 ACATGT A^CATGT
BspMI 1275 GCAGGT ACCTGC(4/8)
BsrDI 1217 GCAATG GCAATG(2/0)
BstXI 1715 CCATGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1160 GGATCT [GA]^GATC[CT]
Bsu36I 1477 CCTGAGG CC^T[ACGT]AGG
DraIII 1736 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 93 TGGCCA [CT]^GGCC[GA]
Eco81I 1477 CCTGAGG CC^T[ACGT]AGG
EcoRV 365 GATATC GAT^ATC
FauI 1389 CCCGC CCCGC(4/6)
HaeIII 94 GGCC GG^CC
MaeI 604 CTAG C^TAG
MfeI 740 CAATTG C^AATTG
MflI 1160 GGATCT [GA]^GATC[CT]
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MspA1I 54 CAGCTG C[AC]G^C[GT]G
MunI 740 CAATTG C^AATTG
NdeI 824 CATATG CA^TATG
PstI 662 CTGCAG CTGCA^G
PvuII 54 CAGCTG CAG^CTG
SapI 505 GAAGAGC GCTCTTC(1/4)
ScaI 572 AGTACT AGT^ACT
SmlI 525 CTCAAG C^T[CT][GA]AG
SnaBI 868 TACGTA TAC^GTA
TaqI 1406 TCGA T^CGA
TthHB8I 1406 TCGA T^CGA
XcmI 1712 CCACCATGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1160 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 370 CCGC CCGC(-3/-1)
1390 CCGC
AseI 238 ATTAAT AT^TAAT
1822 ATTAAT
AsnI 238 ATTAAT AT^TAAT
1822 ATTAAT
BmyI 703 GTGCCC G[AGT]GC[ACT]^C
1030 GGGCAC
BsaAI 472 TACGTG [CT]AC^GT[GA]
868 TACGTA
BsiYI 96 CCACATCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1787 CCTGTTTGGGG
BslI 96 CCACATCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1787 CCTGTTTGGGG
Bsp1286I 703 GTGCCC G[AGT]GC[ACT]^C
1030 GGGCAC
DraII 1277 AGGTCCT [GA]G^G[ACGT]CC[CT]
1365 AGGACCC
EcoO109I 1277 AGGTCCT [GA]G^G[ACGT]CC[CT]
1365 AGGACCC
EcoT14I 181 CCAAGG C^C[AT][AT]GG
916 CCTTGG
MaeII 473 ACGT A^CGT
869 ACGT
MslI 335 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
637 CACCTGGATG
NspI 285 ACATGC [GA]CATG^[CT]
480 ACATGT
PleI 1549 GACTC GAGTC(4/5)
616 GAGTC
PpuMI 1277 AGGTCCT [GA]G^G[AT]CC[CT]
1365 AGGACCC
PshBI 238 ATTAAT AT^TAAT
1822 ATTAAT
StyI 181 CCAAGG C^C[AT][AT]GG
916 CCTTGG
Tsp45I 10 GTCAC ^GT[GC]AC
964 GTCAC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BbvI BsaJI BseRI
BsmAI BsmI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRI EcoRII Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TfiI Tru9I TseI Tsp509I TspRI
XmnI