Restriction map for
pFN21AE1923
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI MvaI
MvnI MwoI NaeI NarI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 814 GTCTAC GT^[AC][GT]AC
AfaI 737 GTAC GT^AC
Asp700I 388 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 662 GAGCAC G[AT]GC[AT]^C
BbvI 258 GCTGC GCAGC(8/12)
BcnI 1119 CCCGG CC^[GC]GG
BmyI 662 GAGCAC G[AGT]GC[ACT]^C
BseRI 1281 GAGGAG GAGGAG(10/8)
BsiHKAI 662 GAGCAC G[AT]GC[AT]^C
BsmAI 1305 GTCTC GTCTC(1/5)
BsmI 722 GCATTC GAATGC(1/-1)
Bsp1286I 662 GAGCAC G[AGT]GC[ACT]^C
BsrDI 1100 GCAATG GCAATG(2/0)
BssKI 1119 CCCGG ^CC[ACGT]GG
BstF5I 1354 CATCC GGATG(2/0)
EcoT14I 841 CCATGG C^C[AT][AT]GG
Fnu4HI 258 GCTGC GC^[ACGT]GC
FokI 1354 CATCC GGATG(9/13)
HapII 1120 CCGG C^CGG
HindIII 1371 AAGCTT A^AGCTT
HpaII 1120 CCGG C^CGG
HphI 757 GGTGA GGTGA(8/7)
ItaI 258 GCTGC GC^[ACGT]GC
MaeII 892 ACGT A^CGT
MslI 763 CATACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1120 CCGG C^CGG
NciI 1119 CCCGG CC^[GC]GG
NcoI 841 CCATGG C^CATGG
NspI 773 GCATGT [GA]CATG^[CT]
PflMI 537 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 737 GTAC GT^AC
SapI 424 GAAGAGC GCTCTTC(1/4)
ScrFI 1119 CCCGG CC^[ACGT]GG
SfaNI 521 GCATC GCATC(5/9)
SmlI 800 CTCAAG C^T[CT][GA]AG
SpeI 616 ACTAGT A^CTAGT
StyI 841 CCATGG C^C[AT][AT]GG
TaqI 861 TCGA T^CGA
TseI 258 GCTGC G^C[AT]GC
Tsp45I 348 GTCAC ^GT[GC]AC
TspRI 267 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 861 TCGA T^CGA
Van91I 537 CCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 388 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AseI 202 ATTAAT AT^TAAT
849 ATTAAT
AsnI 202 ATTAAT AT^TAAT
849 ATTAAT
AspI 161 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
175 GACTTTGTC
BbsI 547 GAAGAC GAAGAC(2/6)
1006 GAAGAC
BclI 57 TGATCA T^GATCA
234 TGATCA
BpuAI 547 GAAGAC GAAGAC(2/6)
1006 GAAGAC
BsaJI 542 CCGTGG C^C[ACGT][ACGT]GG
841 CCATGG
BsiYI 150 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
537 CCAGACCGTGG
BslI 150 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
537 CCAGACCGTGG
BsmFI 159 GGGAC GGGAC(10/14)
1105 GTCCC
Cfr13I 195 GGGCC G^G[ACGT]CC
945 GGGCC
DsaI 542 CCGTGG C^C[GA][CT]GG
841 CCATGG
FbaI 57 TGATCA T^GATCA
234 TGATCA
HinfI 79 GATTC G^A[ACGT]TC
1363 GATTC
MaeIII 348 GTCAC ^GT[ACGT]AC
481 GTAAC
PflFI 161 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
175 GACTTTGTC
PshBI 202 ATTAAT AT^TAAT
849 ATTAAT
Sau96I 195 GGGCC G^G[ACGT]CC
945 GGGCC
SspI 416 AATATT AAT^ATT
1037 AATATT
TfiI 79 GATTC G^A[AT]TC
1363 GATTC
Tth111I 161 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
175 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BpmI BsrI DdeI DpnII EarI
HaeIII Ksp632I MaeI MboI MboII
MnlI MseI NdeII NlaIII Sau3AI
Tru9I Tsp509I