Restriction map for
pFN21AE3243
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanII BbeI BclI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 1328 CGCG CG^CG
AcsI 762 GAATTC [GA]^AATT[CT]
AflII 424 CTTAAG C^TTAAG
AhdI 439 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1597 GTGCAC G^TGCAC
ApaLI 1597 GTGCAC G^TGCAC
ApoI 762 GAATTC [GA]^AATT[CT]
AspEI 439 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1597 GTGCAC G[AT]GC[AT]^C
BbrPI 1594 CACGTG CAC^GTG
BbsI 1416 GAAGAC GAAGAC(2/6)
BcgI 1512 GCAGCTATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1088 GGATAC GTATCC(6/5)
BfrI 424 CTTAAG C^TTAAG
BmyI 1597 GTGCAC G[AGT]GC[ACT]^C
BpuAI 1416 GAAGAC GAAGAC(2/6)
BsaAI 1594 CACGTG [CT]AC^GT[GA]
BsaI 1676 GAGACC GGTCTC(1/5)
BsiHKAI 1597 GTGCAC G[AT]GC[AT]^C
BsmI 945 GAATGC GAATGC(1/-1)
Bsp1286I 1597 GTGCAC G[AGT]GC[ACT]^C
BsrDI 1382 GCAATG GCAATG(2/0)
BssSI 1520 CTCGTG CACGAG(-5/-1)
BstUI 1328 CGCG CG^CG
BstYI 1688 GGATCT [GA]^GATC[CT]
EaeI 413 CGGCCA [CT]^GGCC[GA]
Eam1105I 439 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 768 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 762 GAATTC G^AATTC
HaeII 1668 AGCGCC [GA]GCGC^[CT]
HincII 160 GTTGAC GT[CT]^[GA]AC
HindII 160 GTTGAC GT[CT]^[GA]AC
MflI 1688 GGATCT [GA]^GATC[CT]
MseI 425 TTAA T^TAA
MvnI 1328 CGCG CG^CG
NaeI 411 GCCGGC GCC^GGC
NcoI 782 CCATGG C^CATGG
NdeI 715 CATATG CA^TATG
NgoMIV 411 GCCGGC G^CCGGC
NheI 738 GCTAGC G^CTAGC
PaeR7I 753 CTCGAG C^TCGAG
PmaCI 1594 CACGTG CAC^GTG
PmlI 1594 CACGTG CAC^GTG
PpuMI 683 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 706 CTGCAG CTGCA^G
SfcI 706 CTGCAG C^T[GA][CT]AG
Tru9I 425 TTAA T^TAA
Tsp45I 1465 GTGAC ^GT[GC]AC
XbaI 309 TCTAGA T^CTAGA
XhoI 753 CTCGAG C^TCGAG
XhoII 1688 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 609 GGATC GGATC(4/5)
1688 GGATC
AvaI 753 CTCGAG C^[CT]CG[GA]G
1490 CTCGGG
BsgI 1248 CTGCAC GTGCAG(16/14)
1143 GTGCAG
BsoBI 753 CTCGAG C^[CT]CG[GA]G
1490 CTCGGG
BspMI 1420 ACCTGC ACCTGC(4/8)
1679 ACCTGC
BsrFI 411 GCCGGC [GA]^CCGG[CT]
929 ACCGGC
BstXI 50 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1100 CCACTCTCCTGG
Cfr10I 411 GCCGGC [GA]^CCGG[CT]
929 ACCGGC
DraII 659 AGGCCCC [GA]G^G[ACGT]CC[CT]
683 AGGTCCT
DsaI 89 CCACGG C^C[GA][CT]GG
782 CCATGG
EcoO109I 659 AGGCCCC [GA]G^G[ACGT]CC[CT]
683 AGGTCCT
EcoT14I 782 CCATGG C^C[AT][AT]GG
1274 CCAAGG
MaeIII 1465 GTGAC ^GT[ACGT]AC
1532 GTTAC
MslI 340 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1545 CATCTGCATG
PvuII 502 CAGCTG CAG^CTG
1460 CAGCTG
SmlI 424 CTTAAG C^T[CT][GA]AG
753 CTCGAG
StyI 782 CCATGG C^C[AT][AT]GG
1274 CCAAGG
TfiI 336 GAATC G^A[AT]TC
1456 GATTC
XcmI 219 CCATCGGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1097 CCACCACTCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BbvCI BbvI BcnI BfaI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TspRI
TthHB8I