Restriction map for
pFN21AB7080
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
ClaI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MflI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsaI SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 346 GAATTT [GA]^AATT[CT]
AlwNI 286 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 346 GAATTT [GA]^AATT[CT]
AspI 130 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 161 TGGCCA TGG^CCA
BclI 50 TGATCA T^GATCA
BcnI 78 CCGGG CC^[GC]GG
BglI 68 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 70 CTCCAG CTGGAG(16/14)
BspHI 146 TCATGA T^CATGA
BspMI 95 ACCTGC ACCTGC(4/8)
BsrBI 25 GAGCGG CCGCTC(-3/-3)
BsrDI 230 GCAATG GCAATG(2/0)
BsrFI 338 GCCGGT [GA]^CCGG[CT]
BstAPI 285 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 81 GGTAACC G^GT[ACGT]ACC
BstPI 81 GGTAACC G^GT[ACGT]ACC
Cfr10I 338 GCCGGT [GA]^CCGG[CT]
CpoI 179 CGGACCG CG^G[AT]CCG
DdeI 38 CTGAG C^T[ACGT]AG
DpnII 51 GATC ^GATC
DraII 65 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 117 CCACGG C^C[GA][CT]GG
EaeI 161 TGGCCA [CT]^GGCC[GA]
EcoNI 57 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 65 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 81 GGTAACC G^GT[ACGT]ACC
EcoT14I 333 CCAAGG C^C[AT][AT]GG
FauI 99 GCGGG CCCGC(4/6)
FbaI 50 TGATCA T^GATCA
HgaI 122 GACGC GACGC(5/10)
MboI 51 GATC ^GATC
MluNI 161 TGGCCA TGG^CCA
MscI 161 TGGCCA TGG^CCA
MspA1I 289 CAGCTG C[AC]G^C[GT]G
NciI 78 CCGGG CC^[GC]GG
NdeII 51 GATC ^GATC
NlaIII 147 CATG CATG^
PflFI 130 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 289 CAGCTG CAG^CTG
RcaI 146 TCATGA T^CATGA
RsrII 179 CGGACCG CG^G[AT]CCG
Sau3AI 51 GATC ^GATC
SfiI 67 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 333 CCAAGG C^C[AT][AT]GG
Tsp509I 347 AATT ^AATT
Tth111I 130 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 203 AGCT AG^CT
290 AGCT
BsiYI 57 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
72 CCAGGGCCGGG
BslI 57 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
72 CCAGGGCCGGG
BstNI 72 CCAGG CC^[AT]GG
159 CCTGG
EcoRII 72 CCAGG ^CC[AT]GG
159 CCTGG
HapII 78 CCGG C^CGG
339 CCGG
HpaII 78 CCGG C^CGG
339 CCGG
MaeII 242 ACGT A^CGT
260 ACGT
MspI 78 CCGG C^CGG
339 CCGG
MvaI 72 CCAGG CC^[AT]GG
159 CCTGG
PstI 61 CTGCAG CTGCA^G
292 CTGCAG
SfaNI 139 GATGC GCATC(5/9)
192 GCATC
SfcI 61 CTGCAG C^T[GA][CT]AG
292 CTGCAG
TaqI 137 TCGA T^CGA
274 TCGA
Tsp45I 169 GTCAC ^GT[GC]AC
220 GTGAC
TspRI 17 CAGTG CA[GC]TG[ACGT][ACGT]^
87 CACTG
TthHB8I 137 TCGA T^CGA
274 TCGA
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AvaII BbvI BsaJI BssKI
BstF5I Cfr13I Fnu4HI FokI HaeIII
HphI ItaI MaeIII MnlI MwoI
Sau96I ScrFI TseI