Restriction map for
pFN21AE5153
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FbaI FseI FspI Hin1I HindIII
HinfI HpaI HphI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI MvaI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 97 GTCGAC GT^[AC][GT]AC
AluI 204 AGCT AG^CT
AlwI 81 GGATC GGATC(4/5)
Asp700I 229 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 179 GAGCAC G[AT]GC[AT]^C
AvaI 65 CCCGAG C^[CT]CG[GA]G
AvaII 53 GGACC G^G[AT]CC
BanII 111 GGGCTC G[GA]GC[CT]^C
BbvI 202 GCAGC GCAGC(8/12)
BglI 127 GCCGGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 99 CGACCG CG[GA][CT]^CG
BsiHKAI 179 GAGCAC G[AT]GC[AT]^C
BsiYI 102 CCGGGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 102 CCGGGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 65 CCCGAG C^[CT]CG[GA]G
BsrFI 127 GCCGGT [GA]^CCGG[CT]
BsrI 198 ACTGG ACTGG(1/-1)
BstF5I 117 CATCC GGATG(2/0)
Cfr10I 127 GCCGGT [GA]^CCGG[CT]
DraII 133 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 160 GAAGAG CTCTTC(1/4)
EcoO109I 133 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 109 GCGGG CCCGC(4/6)
FokI 117 CATCC GGATG(9/13)
HaeII 278 AGCGCC [GA]GCGC^[CT]
HaeIII 135 GGCC GG^CC
HgaI 258 GCGTC GACGC(5/10)
HincII 97 GTCGAC GT[CT]^[GA]AC
HindII 97 GTCGAC GT[CT]^[GA]AC
Ksp632I 160 GAAGAG CTCTTC(1/4)
MaeII 28 ACGT A^CGT
MaeIII 7 GTGAC ^GT[ACGT]AC
PstI 262 CTGCAG CTGCA^G
SalI 97 GTCGAC G^TCGAC
SfcI 262 CTGCAG C^T[GA][CT]AG
TaqI 98 TCGA T^CGA
TseI 202 GCAGC G^C[AT]GC
Tsp45I 7 GTGAC ^GT[GC]AC
Tsp509I 273 AATT ^AATT
TspRI 5 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 98 TCGA T^CGA
XmnI 229 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 23 CGCG CG^CG
108 CGCG
BcnI 102 CCGGG CC^[GC]GG
120 CCGGG
BmyI 111 GGGCTC G[AGT]GC[ACT]^C
179 GAGCAC
BsaJI 66 CCGAGG C^C[ACGT][ACGT]GG
102 CCGGGG
Bsp1286I 111 GGGCTC G[AGT]GC[ACT]^C
179 GAGCAC
BssKI 102 CCGGG ^CC[ACGT]GG
120 CCGGG
BstUI 23 CGCG CG^CG
108 CGCG
Cfr13I 53 GGACC G^G[ACGT]CC
134 GGGCC
DpnII 82 GATC ^GATC
294 GATC
Fnu4HI 19 GCGGC GC^[ACGT]GC
202 GCAGC
ItaI 19 GCGGC GC^[ACGT]GC
202 GCAGC
MboI 82 GATC ^GATC
294 GATC
MvnI 23 CGCG CG^CG
108 CGCG
NciI 102 CCGGG CC^[GC]GG
120 CCGGG
NdeII 82 GATC ^GATC
294 GATC
NlaIV 112 GGCTCC GG[ACGT]^[ACGT]CC
133 GGGGCC
Sau3AI 82 GATC ^GATC
294 GATC
Sau96I 53 GGACC G^G[ACGT]CC
134 GGGCC
ScrFI 102 CCGGG CC^[ACGT]GG
120 CCGGG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI Cac8I CfoI HapII HhaI
HinP1I HpaII MboII MnlI MspI
MwoI NlaIII