Restriction map for
pFN21AE1832
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccI AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BamHI BbeI BbvCI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsiEI
BsiHKAI BsiWI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MflI MluI MseI MunI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 894 GACGTC GACGT^C
AccIII 245 TCCGGA T^CCGGA
AcsI 985 AAATTC [GA]^AATT[CT]
AcyI 894 GACGTC G[GA]^CG[CT]C
AflIII 1043 ACACGT A^C[GA][CT]GT
AlwI 147 GATCC GGATC(4/5)
ApoI 985 AAATTC [GA]^AATT[CT]
AvaI 317 CTCGGG C^[CT]CG[GA]G
AvaII 804 GGACC G^G[AT]CC
AviII 851 TGCGCA TGC^GCA
BbrPI 1044 CACGTG CAC^GTG
BcgI 309 GCAAGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 824 CTAG C^TAG
BsaAI 1044 CACGTG [CT]AC^GT[GA]
BsaHI 894 GACGTC G[GA]^CG[CT]C
BsaWI 245 TCCGGA [AT]^CCGG[AT]
BseAI 245 TCCGGA T^CCGGA
BsmAI 121 GAGAC GTCTC(1/5)
BsmBI 121 GAGACG CGTCTC(1/5)
BsmI 613 GAATGC GAATGC(1/-1)
BsoBI 317 CTCGGG C^[CT]CG[GA]G
BspEI 245 TCCGGA T^CCGGA
BspHI 554 TCATGA T^CATGA
BsrBI 9 CCGCTC CCGCTC(-3/-3)
BstAPI 843 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 147 GATC ^GATC
DraIII 625 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 345 CTCTTC CTCTTC(1/4)
EcoT14I 899 CCAAGG C^C[AT][AT]GG
FspI 851 TGCGCA TGC^GCA
HaeII 254 GGCGCT [GA]GCGC^[CT]
Hin1I 894 GACGTC G[GA]^CG[CT]C
HphI 303 GGTGA GGTGA(8/7)
Ksp632I 345 CTCTTC CTCTTC(1/4)
MaeI 824 CTAG C^TAG
MboI 147 GATC ^GATC
MroI 245 TCCGGA T^CCGGA
NdeII 147 GATC ^GATC
PmaCI 1044 CACGTG CAC^GTG
PmlI 1044 CACGTG CAC^GTG
PpuMI 803 AGGACCT [GA]G^G[AT]CC[CT]
PstI 975 CTGCAG CTGCA^G
RcaI 554 TCATGA T^CATGA
Sau3AI 147 GATC ^GATC
SexAI 806 ACCTGGT A^CC[AT]GGT
SfcI 975 CTGCAG C^T[GA][CT]AG
SmlI 136 CTCAAG C^T[CT][GA]AG
StyI 899 CCAAGG C^C[AT][AT]GG
Tsp509I 986 AATT ^AATT
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BalI 83 TGGCCA TGG^CCA
218 TGGCCA
BanII 548 GAGCCC G[GA]GC[CT]^C
747 GGGCTC
BpmI 753 CTCCAG CTGGAG(16/14)
397 CTGGAG
BsgI 160 GTGCAG GTGCAG(16/14)
381 GTGCAG
BsmFI 313 GGGAC GGGAC(10/14)
5 GTCCC
BstXI 722 CCACTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
755 CCAGGGCACTGG
HgaI 306 GACGC GACGC(5/10)
372 GACGC
KspI 204 CCGCGG CCGC^GG
936 CCGCGG
MluNI 83 TGGCCA TGG^CCA
218 TGGCCA
MscI 83 TGGCCA TGG^CCA
218 TGGCCA
NaeI 577 GCCGGC GCC^GGC
1013 GCCGGC
NgoMIV 577 GCCGGC G^CCGGC
1013 GCCGGC
PvuII 214 CAGCTG CAG^CTG
978 CAGCTG
SacII 204 CCGCGG CCGC^GG
936 CCGCGG
SfaNI 461 GATGC GCATC(5/9)
107 GCATC
TaqI 275 TCGA T^CGA
908 TCGA
TthHB8I 275 TCGA T^CGA
908 TCGA
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BbsI BbvI BglI BmyI BpuAI
BsaJI BseRI BsiYI BslI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NlaIII NlaIV
NspI PleI RsaI Sau96I ScrFI
TseI Tsp45I TspRI