Restriction map for
pFN21AE2608
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbrPI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaI BseAI BseRI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SalI SapI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 766 CGCG CG^CG
AcsI 955 AAATTC [GA]^AATT[CT]
AocI 154 CCTCAGG CC^T[ACGT]AGG
Aor51HI 141 AGCGCT AGC^GCT
ApoI 955 AAATTC [GA]^AATT[CT]
BamHI 1030 GGATCC G^GATCC
BbsI 715 GAAGAC GAAGAC(2/6)
BbvCI 277 GCTGAGG CCTCAGC(-5/-2)
BlpI 63 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BpuAI 715 GAAGAC GAAGAC(2/6)
BsaWI 962 ACCGGA [AT]^CCGG[AT]
BsrBI 23 CCGCTC CCGCTC(-3/-3)
BsrDI 347 CATTGC GCAATG(2/0)
BsrFI 173 GCCGGC [GA]^CCGG[CT]
BstAPI 586 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 240 GGTCACC G^GT[ACGT]ACC
BstPI 240 GGTCACC G^GT[ACGT]ACC
BstUI 766 CGCG CG^CG
BstYI 1030 GGATCC [GA]^GATC[CT]
Bsu36I 154 CCTCAGG CC^T[ACGT]AGG
CelII 63 GCTGAGC GC^T[ACGT]AGC
Cfr10I 173 GCCGGC [GA]^CCGG[CT]
EaeI 1013 CGGCCA [CT]^GGCC[GA]
EarI 601 GAAGAG CTCTTC(1/4)
Eco47III 141 AGCGCT AGC^GCT
Eco81I 154 CCTCAGG CC^T[ACGT]AGG
EcoNI 312 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 240 GGTCACC G^GT[ACGT]ACC
Ksp632I 601 GAAGAG CTCTTC(1/4)
KspI 765 CCGCGG CCGC^GG
MaeII 1043 ACGT A^CGT
MflI 1030 GGATCC [GA]^GATC[CT]
MseI 721 TTAA T^TAA
MslI 924 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 765 CCGCGG C[AC]G^C[GT]G
MvnI 766 CGCG CG^CG
NaeI 173 GCCGGC GCC^GGC
NcoI 527 CCATGG C^CATGG
NgoMIV 173 GCCGGC G^CCGGC
NspI 923 GCATGT [GA]CATG^[CT]
PflMI 510 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 496 GAGCTC GAGCT^C
SacII 765 CCGCGG CCGC^GG
SbfI 304 CCTGCAGG CCTGCA^GG
SgrAI 172 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 183 CCCGGG CCC^GGG
Sse8387I 304 CCTGCAGG CCTGCA^GG
Tru9I 721 TTAA T^TAA
Tsp509I 956 AATT ^AATT
TspRI 780 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 510 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1030 GGATCC [GA]^GATC[CT]
XmaCI 183 CCCGGG C^CCGGG
XmaI 183 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 180 GGGCCC GGGCC^C
201 GGGCCC
AspHI 412 GAGCAC G[AT]GC[AT]^C
496 GAGCTC
BanI 76 GGCGCC G^G[CT][GA]CC
552 GGCGCC
BbeI 76 GGCGCC GGCGC^C
552 GGCGCC
BglI 68 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
173 GCCGGCGGGGC
BsgI 442 CTGCAC GTGCAG(16/14)
876 CTGCAC
BsiHKAI 412 GAGCAC G[AT]GC[AT]^C
496 GAGCTC
BsmAI 420 GAGAC GTCTC(1/5)
1009 GAGAC
BsmBI 420 GAGACG CGTCTC(1/5)
1009 GAGACG
BstXI 139 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
542 CCACCAGCCTGG
DpnII 612 GATC ^GATC
1031 GATC
DsaI 527 CCATGG C^C[GA][CT]GG
765 CCGCGG
FauI 177 GCGGG CCCGC(4/6)
864 GCGGG
HgaI 150 GACGC GACGC(5/10)
422 GACGC
KasI 76 GGCGCC G^GCGCC
552 GGCGCC
MboI 612 GATC ^GATC
1031 GATC
NarI 76 GGCGCC GG^CGCC
552 GGCGCC
NdeII 612 GATC ^GATC
1031 GATC
PleI 300 GAGTC GAGTC(4/5)
604 GAGTC
PstI 305 CTGCAG CTGCA^G
316 CTGCAG
Sau3AI 612 GATC ^GATC
1031 GATC
SfcI 305 CTGCAG C^T[GA][CT]AG
316 CTGCAG
SfoI 76 GGCGCC GGC^GCC
552 GGCGCC
TaqI 698 TCGA T^CGA
914 TCGA
TfiI 117 GAATC G^A[AT]TC
535 GAATC
Tsp45I 241 GTCAC ^GT[GC]AC
622 GTCAC
TthHB8I 698 TCGA T^CGA
914 TCGA
XcmI 219 CCAGGCCCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
677 CCACGCTGGACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PpuMI Sau96I
ScrFI SfaNI StuI StyI TseI