Restriction map for
pFN21AE3119
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvrII BalI BamHI BanI BbeI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaHI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DpnII DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI Sau3AI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AfaI 720 GTAC GT^AC
ApaI 739 GGGCCC GGGCC^C
Asp700I 225 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 266 TGCGCA TGC^GCA
BbrPI 79 CACGTG CAC^GTG
BcgI 758 GCAGCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 472 CTAG C^TAG
BlpI 808 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 808 GCTCAGC GC^T[ACGT]AGC
BsaAI 79 CACGTG [CT]AC^GT[GA]
BsaI 645 GGTCTC GGTCTC(1/5)
BsiEI 484 CGGCCG CG[GA][CT]^CG
BsmAI 646 GTCTC GTCTC(1/5)
BspHI 149 TCATGA T^CATGA
CelII 808 GCTCAGC GC^T[ACGT]AGC
CfoI 267 GCGC GCG^C
DrdI 376 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 484 CGGCCG C^GGCCG
EclXI 484 CGGCCG C^GGCCG
Eco52I 484 CGGCCG C^GGCCG
EcoNI 174 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 194 CCAAGG C^C[AT][AT]GG
FspI 266 TGCGCA TGC^GCA
HhaI 267 GCGC GCG^C
HinP1I 267 GCGC G^CGC
HinfI 746 GAGTC G^A[ACGT]TC
MaeI 472 CTAG C^TAG
MaeII 80 ACGT A^CGT
MslI 75 CACGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 746 GAGTC GAGTC(4/5)
PmaCI 79 CACGTG CAC^GTG
PmlI 79 CACGTG CAC^GTG
PpuMI 134 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 639 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 120 CTGCAG CTGCA^G
RcaI 149 TCATGA T^CATGA
RsaI 720 GTAC GT^AC
ScaI 719 AGTACT AGT^ACT
SfaNI 4 GATGC GCATC(5/9)
SfcI 120 CTGCAG C^T[GA][CT]AG
SmaI 671 CCCGGG CCC^GGG
SmlI 421 CTCAAG C^T[CT][GA]AG
StuI 794 AGGCCT AGG^CCT
StyI 194 CCAAGG C^C[AT][AT]GG
Tsp509I 306 AATT ^AATT
TspRI 619 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 17 CCAAAGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 671 CCCGGG C^CCGGG
XmaI 671 CCCGGG C^CCGGG
XmnI 225 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 94 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
123 CAGCCTCTG
BcnI 671 CCCGG CC^[GC]GG
672 CCGGG
BpmI 64 CTGGAG CTGGAG(16/14)
350 CTGGAG
BsgI 534 CTGCAC GTGCAG(16/14)
553 GTGCAG
DdeI 660 CTCAG C^T[ACGT]AG
809 CTCAG
DraII 134 GGGACCT [GA]G^G[ACGT]CC[CT]
739 GGGCCCC
DsaI 212 CCGTGG C^C[GA][CT]GG
357 CCACGG
EaeI 215 TGGCCG [CT]^GGCC[GA]
484 CGGCCG
EarI 691 CTCTTC CTCTTC(1/4)
283 GAAGAG
EcoO109I 134 GGGACCT [GA]G^G[ACGT]CC[CT]
739 GGGCCCC
HphI 191 TCACC GGTGA(8/7)
749 TCACC
Ksp632I 691 CTCTTC CTCTTC(1/4)
283 GAAGAG
MseI 38 TTAA T^TAA
500 TTAA
NciI 671 CCCGG CC^[GC]GG
672 CCGGG
TaqI 143 TCGA T^CGA
593 TCGA
Tru9I 38 TTAA T^TAA
500 TTAA
TthHB8I 143 TCGA T^CGA
593 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV PflMI PvuII Sau96I
ScrFI TseI Tsp45I Van91I