Restriction map for
pFN21AE1641
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DpnII DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HphI KasI KpnI KspI
MaeI MaeII MaeIII MamI MboI
MfeI MflI MluI MroI MslI
MunI NaeI NarI NcoI NdeII
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI Sau3AI ScaI SexAI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
ApaI 337 GGGCCC GGGCC^C
AvaI 359 CCCGGG C^[CT]CG[GA]G
AvaII 317 GGACC G^G[AT]CC
BalI 368 TGGCCA TGG^CCA
BanII 337 GGGCCC G[GA]GC[CT]^C
BbsI 444 GAAGAC GAAGAC(2/6)
BglI 227 GCCCCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 337 GGGCCC G[AGT]GC[ACT]^C
BpuAI 444 GAAGAC GAAGAC(2/6)
BsaI 61 GAGACC GGTCTC(1/5)
BseRI 453 CTCCTC GAGGAG(10/8)
BsiEI 234 CGGCCG CG[GA][CT]^CG
BsmI 419 GAATGC GAATGC(1/-1)
BsoBI 359 CCCGGG C^[CT]CG[GA]G
Bsp1286I 337 GGGCCC G[AGT]GC[ACT]^C
BsrFI 232 ACCGGC [GA]^CCGG[CT]
BssHII 406 GCGCGC G^CGCGC
Cfr10I 232 ACCGGC [GA]^CCGG[CT]
DraII 337 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 194 CCACGG C^C[GA][CT]GG
EagI 234 CGGCCG C^GGCCG
EclXI 234 CGGCCG C^GGCCG
Eco52I 234 CGGCCG C^GGCCG
EcoO109I 337 GGGCCCC [GA]G^G[ACGT]CC[CT]
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MseI 34 TTAA T^TAA
NdeI 250 CATATG CA^TATG
PshAI 63 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 426 CTGCAG CTGCA^G
PvuII 18 CAGCTG CAG^CTG
SapI 105 GAAGAGC GCTCTTC(1/4)
SbfI 425 CCTGCAGG CCTGCA^GG
SfaNI 150 GATGC GCATC(5/9)
SfcI 426 CTGCAG C^T[GA][CT]AG
SfiI 226 GGCCCCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 359 CCCGGG CCC^GGG
Sse8387I 425 CCTGCAGG CCTGCA^GG
Tru9I 34 TTAA T^TAA
TspRI 330 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 359 CCCGGG C^CCGGG
XmaI 359 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AccII 405 CGCG CG^CG
407 CGCG
Asp700I 432 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
444 GAAGACCTTC
BsmAI 61 GAGAC GTCTC(1/5)
239 GAGAC
BsrI 135 CCAGT ACTGG(1/-1)
329 CCAGT
BstUI 405 CGCG CG^CG
407 CGCG
Cac8I 245 GCGAGC GC[ACGT]^[ACGT]GC
406 GCGCGC
EarI 58 GAAGAG CTCTTC(1/4)
105 GAAGAG
Ksp632I 58 GAAGAG CTCTTC(1/4)
105 GAAGAG
MspA1I 18 CAGCTG C[AC]G^C[GT]G
203 CCGCTG
MvnI 405 CGCG CG^CG
407 CGCG
XmnI 432 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
444 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BpmI
BsaJI BsiYI BslI BsmFI BssKI
BstNI CfoI Cfr13I EaeI EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI TseI