Restriction map for
pFN21AE5740
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AclI AflIII AhdI Alw44I
AlwNI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BcgI BclI BlnI BlpI
Bpu1102I BsaBI BsiHKAI BsiWI BsmBI
BspDI BspHI BspLU11I BsrDI BssHII
BssSI BstBI BstEII BstPI CelII
ClaI CpoI DrdI Eam1105I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII KpnI
KspI MamI MfeI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1756 GACGTC GACGT^C
AccI 504 GTATAC GT^[AC][GT]AC
AccIII 1670 TCCGGA T^CCGGA
AflII 1948 CTTAAG C^TTAAG
AgeI 342 ACCGGT A^CCGGT
Aor51HI 1183 AGCGCT AGC^GCT
Asp700I 1063 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 438 TGGCCA TGG^CCA
BamHI 916 GGATCC G^GATCC
BanI 137 GGCGCC G^G[CT][GA]CC
BbeI 137 GGCGCC GGCGC^C
BbrPI 1288 CACGTG CAC^GTG
BbvCI 84 CCTCAGC CCTCAGC(-5/-2)
BfrI 1948 CTTAAG C^TTAAG
BglII 1560 AGATCT A^GATCT
BsaAI 1288 CACGTG [CT]AC^GT[GA]
BsaI 228 GAGACC GGTCTC(1/5)
BseAI 1670 TCCGGA T^CCGGA
BsgI 445 GTGCAG GTGCAG(16/14)
BsmI 1510 GCATTC GAATGC(1/-1)
Bsp1407I 1367 TGTACA T^GTACA
BspEI 1670 TCCGGA T^CCGGA
BspMI 447 GCAGGT ACCTGC(4/8)
BsrBI 1302 CCGCTC CCGCTC(-3/-3)
BsrGI 1367 TGTACA T^GTACA
Bst1107I 504 GTATAC GTA^TAC
BstAPI 1414 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 504 GTATAC GTA^TAC
DraI 2005 TTTAAA TTT^AAA
DraIII 1937 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 353 CCGTGG C^C[GA][CT]GG
Eco47III 1183 AGCGCT AGC^GCT
EcoT14I 471 CCAAGG C^C[AT][AT]GG
HpaI 828 GTTAAC GTT^AAC
KasI 137 GGCGCC G^GCGCC
MluNI 438 TGGCCA TGG^CCA
MroI 1670 TCCGGA T^CCGGA
MscI 438 TGGCCA TGG^CCA
NarI 137 GGCGCC GG^CGCC
PaeR7I 312 CTCGAG C^TCGAG
PinAI 342 ACCGGT A^CCGGT
PmaCI 1288 CACGTG CAC^GTG
PmlI 1288 CACGTG CAC^GTG
PpuMI 1646 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1591 CTGCAG CTGCA^G
SapI 1888 GAAGAGC GCTCTTC(1/4)
SfoI 137 GGCGCC GGC^GCC
SgrAI 341 CACCGGTG C[GA]^CCGG[CT]G
SspBI 1367 TGTACA T^GTACA
StuI 819 AGGCCT AGG^CCT
StyI 471 CCAAGG C^C[AT][AT]GG
TfiI 304 GAATC G^A[AT]TC
XhoI 312 CTCGAG C^TCGAG
XmnI 1063 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 137 GGCGCC G[GA]^CG[CT]C
1756 GACGTC
AocI 1092 CCTGAGG CC^T[ACGT]AGG
2047 CCTGAGG
ApaI 357 GGGCCC GGGCC^C
1777 GGGCCC
BbsI 2078 GAAGAC GAAGAC(2/6)
977 GTCTTC
BciVI 1765 GGATAC GTATCC(6/5)
524 GTATCC
BglI 163 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
431 GCCTCTATGGC
BpmI 183 CTGGAG CTGGAG(16/14)
2015 CTGGAG
BpuAI 2078 GAAGAC GAAGAC(2/6)
977 GTCTTC
BsaHI 137 GGCGCC G[GA]^CG[CT]C
1756 GACGTC
BsaWI 342 ACCGGT [AT]^CCGG[AT]
1670 TCCGGA
BsmAI 228 GAGAC GTCTC(1/5)
1278 GTCTC
BstXI 921 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCAGCCACGTGG
Bsu36I 1092 CCTGAGG CC^T[ACGT]AGG
2047 CCTGAGG
DraII 357 GGGCCCC [GA]G^G[ACGT]CC[CT]
1646 AGGTCCC
EagI 170 CGGCCG C^GGCCG
363 CGGCCG
EclXI 170 CGGCCG C^GGCCG
363 CGGCCG
Eco52I 170 CGGCCG C^GGCCG
363 CGGCCG
Eco81I 1092 CCTGAGG CC^T[ACGT]AGG
2047 CCTGAGG
EcoO109I 357 GGGCCCC [GA]G^G[ACGT]CC[CT]
1646 AGGTCCC
HaeII 137 GGCGCC [GA]GCGC^[CT]
1183 AGCGCT
Hin1I 137 GGCGCC G[GA]^CG[CT]C
1756 GACGTC
HphI 1089 TCACC GGTGA(8/7)
1871 TCACC
MspA1I 1588 CAGCTG C[AC]G^C[GT]G
1725 CAGCTG
PvuII 1588 CAGCTG CAG^CTG
1725 CAGCTG
SfcI 1591 CTGCAG C^T[GA][CT]AG
1830 CTGTAG
SspI 770 AATATT AAT^ATT
1054 AATATT
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII