Restriction map for
pFN21AE2616
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BseRI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 418 GACGTC GACGT^C
AcsI 595 GAATTC [GA]^AATT[CT]
AflIII 911 ACACGT A^C[GA][CT]GT
AhdI 172 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1102 GTGCAC G^TGCAC
AlwI 951 GATCC GGATC(4/5)
ApaLI 1102 GTGCAC G^TGCAC
ApoI 595 GAATTC [GA]^AATT[CT]
AspEI 172 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 535 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1221 TGGCCA TGG^CCA
BbeI 186 GGCGCC GGCGC^C
BbrPI 409 CACGTG CAC^GTG
BglI 373 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 54 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 54 GCTGAGC GC^T[ACGT]AGC
BsaI 1278 GGTCTC GGTCTC(1/5)
BsgI 1293 CTGCAC GTGCAG(16/14)
BsiEI 753 CGACCG CG[GA][CT]^CG
BsmBI 902 GAGACG CGTCTC(1/5)
Bsp1407I 611 TGTACA T^GTACA
BspMI 699 GCAGGT ACCTGC(4/8)
BsrBI 289 CCGCTC CCGCTC(-3/-3)
BsrGI 611 TGTACA T^GTACA
BssSI 442 CACGAG CACGAG(-5/-1)
BstEII 13 GGTGACC G^GT[ACGT]ACC
BstPI 13 GGTGACC G^GT[ACGT]ACC
BstYI 950 AGATCC [GA]^GATC[CT]
CelII 54 GCTGAGC GC^T[ACGT]AGC
DdeI 55 CTGAG C^T[ACGT]AG
DraIII 1225 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 172 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 13 GGTGACC G^GT[ACGT]ACC
EcoRI 595 GAATTC G^AATTC
HgaI 557 GCGTC GACGC(5/10)
HincII 1132 GTCAAC GT[CT]^[GA]AC
HindII 1132 GTCAAC GT[CT]^[GA]AC
KasI 186 GGCGCC G^GCGCC
MflI 950 AGATCC [GA]^GATC[CT]
MluNI 1221 TGGCCA TGG^CCA
MscI 1221 TGGCCA TGG^CCA
MslI 495 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 183 GCCGGC GCC^GGC
NarI 186 GGCGCC GG^CGCC
NgoMIV 183 GCCGGC G^CCGGC
NspI 325 ACATGC [GA]CATG^[CT]
PflFI 535 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 409 CACGTG CAC^GTG
PmlI 409 CACGTG CAC^GTG
PshAI 414 GACCGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 186 GGCGCC GGC^GCC
SgrAI 182 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1201 CCCGGG CCC^GGG
SspBI 611 TGTACA T^GTACA
TaqI 170 TCGA T^CGA
Tsp509I 596 AATT ^AATT
Tth111I 535 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 170 TCGA T^CGA
XhoII 950 AGATCC [GA]^GATC[CT]
XmaCI 1201 CCCGGG C^CCGGG
XmaI 1201 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 186 GGCGCC G[GA]^CG[CT]C
418 GACGTC
BsaAI 409 CACGTG [CT]AC^GT[GA]
912 CACGTA
BsaHI 186 GGCGCC G[GA]^CG[CT]C
418 GACGTC
BsmAI 902 GAGAC GTCTC(1/5)
1279 GTCTC
HaeII 186 GGCGCC [GA]GCGC^[CT]
1264 AGCGCC
Hin1I 186 GGCGCC G[GA]^CG[CT]C
418 GACGTC
HinfI 1012 GACTC G^A[ACGT]TC
1050 GAGTC
MboII 297 GAAGA GAAGA(8/7)
885 GAAGA
MspA1I 633 CAGCTG C[AC]G^C[GT]G
1138 CAGCTG
PflMI 1137 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1269 CCAGTCCATGG
PleI 1012 GACTC GAGTC(4/5)
1050 GAGTC
PpuMI 1285 GGGTCCC [GA]G^G[AT]CC[CT]
1336 AGGACCT
PvuII 633 CAGCTG CAG^CTG
1138 CAGCTG
SacI 445 GAGCTC GAGCT^C
804 GAGCTC
SfcI 1039 CTACAG C^T[GA][CT]AG
1057 CTACAG
Van91I 1137 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1269 CCAGTCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MnlI MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I
TspRI