Restriction map for
pFN21AE6795
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BbeI BbvCI BfrI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 399 GTAC GT^AC
AflIII 1236 ACACGT A^C[GA][CT]GT
AhdI 1235 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1235 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1091 CTCGGG C^[CT]CG[GA]G
AvaII 1242 GGTCC G^G[AT]CC
BamHI 305 GGATCC G^GATCC
BanI 1260 GGTGCC G^G[CT][GA]CC
BbrPI 1237 CACGTG CAC^GTG
BcgI 1439 CGATCTTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 911 GGATAC GTATCC(6/5)
BfaI 205 CTAG C^TAG
BsaAI 1237 CACGTG [CT]AC^GT[GA]
BsaI 95 GGTCTC GGTCTC(1/5)
BsgI 554 CTGCAC GTGCAG(16/14)
BsmBI 538 GAGACG CGTCTC(1/5)
BsoBI 1091 CTCGGG C^[CT]CG[GA]G
BspHI 689 TCATGA T^CATGA
BspMI 1002 GCAGGT ACCTGC(4/8)
BstEII 1137 GGTGACC G^GT[ACGT]ACC
BstPI 1137 GGTGACC G^GT[ACGT]ACC
DraII 318 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 961 CCGCGG C^C[GA][CT]GG
Eam1105I 1235 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 25 GAAGAG CTCTTC(1/4)
EcoO109I 318 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1137 GGTGACC G^GT[ACGT]ACC
FauI 960 CCCGC CCCGC(4/6)
Ksp632I 25 GAAGAG CTCTTC(1/4)
KspI 961 CCGCGG CCGC^GG
MaeI 205 CTAG C^TAG
PmaCI 1237 CACGTG CAC^GTG
PmlI 1237 CACGTG CAC^GTG
PstI 1066 CTGCAG CTGCA^G
RcaI 689 TCATGA T^CATGA
RsaI 399 GTAC GT^AC
SacII 961 CCGCGG CCGC^GG
SapI 25 GAAGAGC GCTCTTC(1/4)
SfcI 1066 CTGCAG C^T[GA][CT]AG
SmlI 1396 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 962 CGCG CG^CG
1685 CGCG
AlwNI 889 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1003 CAGGTGCTG
ApaI 318 GGGCCC GGGCC^C
803 GGGCCC
BalI 773 TGGCCA TGG^CCA
1402 TGGCCA
BclI 506 TGATCA T^GATCA
662 TGATCA
BlpI 337 GCTGAGC GC^T[ACGT]AGC
1507 GCTGAGC
Bpu1102I 337 GCTGAGC GC^T[ACGT]AGC
1507 GCTGAGC
BsrI 884 ACTGG ACTGG(1/-1)
435 CCAGT
BstUI 962 CGCG CG^CG
1685 CGCG
Cac8I 1263 GCCTGC GC[ACGT]^[ACGT]GC
1352 GCTTGC
CelII 337 GCTGAGC GC^T[ACGT]AGC
1507 GCTGAGC
DraIII 262 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
777 CACTGAGTG
EcoT14I 412 CCTTGG C^C[AT][AT]GG
1142 CCTTGG
FbaI 506 TGATCA T^GATCA
662 TGATCA
MluNI 773 TGGCCA TGG^CCA
1402 TGGCCA
MscI 773 TGGCCA TGG^CCA
1402 TGGCCA
MslI 564 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1254 CATGGAGGTG
MvnI 962 CGCG CG^CG
1685 CGCG
NspI 388 ACATGC [GA]CATG^[CT]
460 ACATGC
PflMI 776 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1340 CCATCATCTGG
SacI 28 GAGCTC GAGCT^C
1510 GAGCTC
StyI 412 CCTTGG C^C[AT][AT]GG
1142 CCTTGG
Van91I 776 CCACTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1340 CCATCATCTGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AspHI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI BstXI BstYI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I TspRI XcmI XhoII