Restriction map for
pFN21AE6791
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1544 GTAGAC GT^[AC][GT]AC
AclI 856 AACGTT AA^CGTT
Alw44I 172 GTGCAC G^TGCAC
ApaLI 172 GTGCAC G^TGCAC
Asp700I 1647 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 172 GTGCAC G[AT]GC[AT]^C
BanI 2124 GGCACC G^G[CT][GA]CC
BanII 163 GAGCCC G[GA]GC[CT]^C
BbsI 1135 GAAGAC GAAGAC(2/6)
BclI 293 TGATCA T^GATCA
BglII 2003 AGATCT A^GATCT
BpuAI 1135 GAAGAC GAAGAC(2/6)
BsaBI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 959 GAGACC GGTCTC(1/5)
BseRI 74 GAGGAG GAGGAG(10/8)
BsiHKAI 172 GTGCAC G[AT]GC[AT]^C
BsmAI 959 GAGAC GTCTC(1/5)
BspLU11I 2280 ACATGT A^CATGT
BspMI 2065 ACCTGC ACCTGC(4/8)
BsrDI 1583 GCAATG GCAATG(2/0)
BssSI 1174 CACGAG CACGAG(-5/-1)
BstXI 1173 CCACGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1209 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 483 CCATGG C^C[GA][CT]GG
EcoNI 441 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1246 ATGCAT ATGCA^T
FbaI 293 TGATCA T^GATCA
HincII 672 GTTAAC GT[CT]^[GA]AC
HindII 672 GTTAAC GT[CT]^[GA]AC
HindIII 1069 AAGCTT A^AGCTT
HpaI 672 GTTAAC GTT^AAC
MamI 2004 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 709 CAATTG C^AATTG
MspA1I 766 CAGCGG C[AC]G^C[GT]G
MunI 709 CAATTG C^AATTG
NcoI 483 CCATGG C^CATGG
NdeI 1799 CATATG CA^TATG
NsiI 1246 ATGCAT ATGCA^T
PflMI 1916 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 508 GAGTC GAGTC(4/5)
PpuMI 500 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 856 AACGTT AA^CGTT
PstI 1284 CTGCAG CTGCA^G
TaqI 1017 TCGA T^CGA
TthHB8I 1017 TCGA T^CGA
Van91I 1916 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 117 TCTAGA T^CTAGA
XmnI 1647 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 1273 AAATTT [GA]^AATT[CT]
1519 AAATTT
AflIII 1669 ACGTGT A^C[GA][CT]GT
2280 ACATGT
ApoI 1273 AAATTT [GA]^AATT[CT]
1519 AAATTT
BalI 228 TGGCCA TGG^CCA
1707 TGGCCA
DraII 244 AGGCCCT [GA]G^G[ACGT]CC[CT]
500 GGGTCCC
EaeI 228 TGGCCA [CT]^GGCC[GA]
1707 TGGCCA
EcoO109I 244 AGGCCCT [GA]G^G[ACGT]CC[CT]
500 GGGTCCC
EcoRV 806 GATATC GAT^ATC
1822 GATATC
MaeII 857 ACGT A^CGT
1669 ACGT
MluNI 228 TGGCCA TGG^CCA
1707 TGGCCA
MscI 228 TGGCCA TGG^CCA
1707 TGGCCA
ScaI 1309 AGTACT AGT^ACT
1373 AGTACT
SphI 435 GCATGC GCATG^C
1248 GCATGC
SspI 799 AATATT AAT^ATT
1298 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmFI BsmI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII