Restriction map for
pFN21AE6463
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HapII
HgaI Hin1I HindIII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 534 GTAC GT^AC
AflIII 233 ACATGT A^C[GA][CT]GT
AvaII 86 GGACC G^G[AT]CC
AvrII 381 CCTAGG C^CTAGG
BalI 242 TGGCCA TGG^CCA
BamHI 1213 GGATCC G^GATCC
BanII 35 GGGCTC G[GA]GC[CT]^C
BlnI 381 CCTAGG C^CTAGG
BmyI 35 GGGCTC G[AGT]GC[ACT]^C
BsaI 489 GGTCTC GGTCTC(1/5)
BsmBI 103 CGTCTC CGTCTC(1/5)
BsmFI 85 GGGAC GGGAC(10/14)
Bsp1286I 35 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 233 ACATGT A^CATGT
BssSI 177 CACGAG CACGAG(-5/-1)
BstAPI 683 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 707 CCAAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 85 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 875 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1249 CCATGG C^C[GA][CT]GG
EaeI 242 TGGCCA [CT]^GGCC[GA]
EcoO109I 85 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1032 GATATC GAT^ATC
FauI 346 GCGGG CCCGC(4/6)
HincII 761 GTCAAC GT[CT]^[GA]AC
HindII 761 GTCAAC GT[CT]^[GA]AC
MaeII 102 ACGT A^CGT
MfeI 1151 CAATTG C^AATTG
MluNI 242 TGGCCA TGG^CCA
MscI 242 TGGCCA TGG^CCA
MspA1I 344 CAGCGG C[AC]G^C[GT]G
MunI 1151 CAATTG C^AATTG
NcoI 1249 CCATGG C^CATGG
PpuMI 85 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1289 CTGCAG CTGCA^G
RsaI 534 GTAC GT^AC
SfcI 1289 CTGCAG C^T[GA][CT]AG
SspI 801 AATATT AAT^ATT
Tsp45I 1066 GTCAC ^GT[GC]AC
XcmI 736 CCACTGATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 1037 CCGC CCGC(-3/-1)
346 GCGG
AcsI 914 GAATTT [GA]^AATT[CT]
1091 AAATTC
ApoI 914 GAATTT [GA]^AATT[CT]
1091 AAATTC
BsrI 587 ACTGG ACTGG(1/-1)
1075 ACTGG
BssKI 282 CCTGG ^CC[ACGT]GG
1053 CCTGG
BstNI 282 CCTGG CC^[AT]GG
1053 CCTGG
CfoI 189 GCGC GCG^C
695 GCGC
EcoNI 76 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
852 CCTTAGTCAGG
EcoRII 282 CCTGG ^CC[AT]GG
1053 CCTGG
HhaI 189 GCGC GCG^C
695 GCGC
HinP1I 189 GCGC G^CGC
695 GCGC
HinfI 298 GAATC G^A[ACGT]TC
814 GATTC
MaeIII 370 GTTAC ^GT[ACGT]AC
1066 GTCAC
MvaI 282 CCTGG CC^[AT]GG
1053 CCTGG
NspI 233 ACATGT [GA]CATG^[CT]
890 ACATGC
ScrFI 282 CCTGG CC^[ACGT]GG
1053 CCTGG
TfiI 298 GAATC G^A[AT]TC
814 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AlwI AlwNI BbvI BfaI
BsaJI BsgI BsiYI BslI BsmAI
BstF5I BstYI Cac8I Cfr13I DdeI
DpnII EcoT14I Fnu4HI FokI HaeIII
ItaI MaeI MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I SfaNI
StyI Tru9I TseI Tsp509I TspRI
XhoII