Restriction map for
pFN21AE6445
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I FauI FbaI
FseI FspI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MamI MluI MluNI
MroI MscI MspA1I MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflIII 654 ACATGT A^C[GA][CT]GT
Alw44I 449 GTGCAC G^TGCAC
AlwI 759 GGATC GGATC(4/5)
ApaLI 449 GTGCAC G^TGCAC
Asp700I 304 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 449 GTGCAC G[AT]GC[AT]^C
BciVI 686 GGATAC GTATCC(6/5)
BpmI 1166 CTGGAG CTGGAG(16/14)
BsaI 1527 GAGACC GGTCTC(1/5)
BseRI 202 GAGGAG GAGGAG(10/8)
BsiHKAI 449 GTGCAC G[AT]GC[AT]^C
BspLU11I 654 ACATGT A^CATGT
BstAPI 290 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 759 GGATCT [GA]^GATC[CT]
Cac8I 271 GCCAGC GC[ACGT]^[ACGT]GC
DraIII 143 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 184 GAATTC G^AATTC
EcoT22I 297 ATGCAT ATGCA^T
HgaI 1066 GCGTC GACGC(5/10)
HindIII 1468 AAGCTT A^AGCTT
HpaI 720 GTTAAC GTT^AAC
HphI 171 TCACC GGTGA(8/7)
MaeII 1478 ACGT A^CGT
MfeI 1107 CAATTG C^AATTG
MflI 759 GGATCT [GA]^GATC[CT]
MslI 286 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1107 CAATTG C^AATTG
NsiI 297 ATGCAT ATGCA^T
PflMI 1317 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 307 GCTCTTC GCTCTTC(1/4)
SfcI 1187 CTACAG C^T[GA][CT]AG
SmlI 1252 CTTGAG C^T[CT][GA]AG
Van91I 1317 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 238 CCAGAACTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 759 GGATCT [GA]^GATC[CT]
XmnI 304 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 403 GTATAC GT^[AC][GT]AC
481 GTATAC
AciI 1334 CCGC CCGC(-3/-1)
1445 CCGC
AfaI 890 GTAC GT^AC
1073 GTAC
AlwNI 601 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1337 CAGCGACTG
BanII 797 GAGCCC G[GA]GC[CT]^C
1235 GAGCCC
BsrDI 824 CATTGC GCAATG(2/0)
854 CATTGC
BssKI 135 CCAGG ^CC[ACGT]GG
1058 CCAGG
Bst1107I 403 GTATAC GTA^TAC
481 GTATAC
BstNI 135 CCAGG CC^[AT]GG
1058 CCAGG
BstZ17I 403 GTATAC GTA^TAC
481 GTATAC
DpnII 760 GATC ^GATC
1207 GATC
DsaI 431 CCATGG C^C[GA][CT]GG
1317 CCATGG
EcoRII 135 CCAGG ^CC[AT]GG
1058 CCAGG
EcoRV 641 GATATC GAT^ATC
1029 GATATC
EcoT14I 431 CCATGG C^C[AT][AT]GG
1317 CCATGG
MboI 760 GATC ^GATC
1207 GATC
MvaI 135 CCAGG CC^[AT]GG
1058 CCAGG
NcoI 431 CCATGG C^CATGG
1317 CCATGG
NdeII 760 GATC ^GATC
1207 GATC
RsaI 890 GTAC GT^AC
1073 GTAC
Sau3AI 760 GATC ^GATC
1207 GATC
ScrFI 135 CCAGG CC^[ACGT]GG
1058 CCAGG
StyI 431 CCATGG C^C[AT][AT]GG
1317 CCATGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AluI ApoI AvaII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BstF5I
Cfr13I DdeI EarI Fnu4HI FokI
HincII HindII HinfI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MwoI NlaIII NlaIV NspI Sau96I
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I