Restriction map for
pFN21AE6396
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV FseI FspI HaeII
HapII HgaI Hin1I HpaI HpaII
KasI KpnI KspI MamI MluI
MroI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 392 GTCTAC GT^[AC][GT]AC
AciI 572 GCGG CCGC(-3/-1)
AlwI 196 GATCC GGATC(4/5)
AocI 604 CCTGAGG CC^T[ACGT]AGG
AspHI 373 GTGCTC G[AT]GC[AT]^C
BanI 402 GGCACC G^G[CT][GA]CC
BbrPI 639 CACGTG CAC^GTG
BbsI 90 GAAGAC GAAGAC(2/6)
BbvI 1077 GCTGC GCAGC(8/12)
BglII 233 AGATCT A^GATCT
BpmI 397 CTCCAG CTGGAG(16/14)
BpuAI 90 GAAGAC GAAGAC(2/6)
BsaAI 639 CACGTG [CT]AC^GT[GA]
BsgI 636 CTGCAC GTGCAG(16/14)
BsiHKAI 373 GTGCTC G[AT]GC[AT]^C
BspHI 743 TCATGA T^CATGA
BspMI 634 ACCTGC ACCTGC(4/8)
BsrDI 599 CATTGC GCAATG(2/0)
BstEII 270 GGTGACC G^GT[ACGT]ACC
BstPI 270 GGTGACC G^GT[ACGT]ACC
BstXI 260 CCATAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 604 CCTGAGG CC^T[ACGT]AGG
CfoI 610 GCGC GCG^C
Eco81I 604 CCTGAGG CC^T[ACGT]AGG
EcoO65I 270 GGTGACC G^GT[ACGT]ACC
EcoT14I 1172 CCAAGG C^C[AT][AT]GG
EcoT22I 409 ATGCAT ATGCA^T
FauI 572 GCGGG CCCGC(4/6)
Fnu4HI 1077 GCTGC GC^[ACGT]GC
HhaI 610 GCGC GCG^C
HinP1I 610 GCGC G^CGC
HincII 825 GTCAAC GT[CT]^[GA]AC
HindII 825 GTCAAC GT[CT]^[GA]AC
ItaI 1077 GCTGC GC^[ACGT]GC
MaeII 640 ACGT A^CGT
MfeI 857 CAATTG C^AATTG
MunI 857 CAATTG C^AATTG
NsiI 409 ATGCAT ATGCA^T
PleI 338 GACTC GAGTC(4/5)
PmaCI 639 CACGTG CAC^GTG
PmlI 639 CACGTG CAC^GTG
RcaI 743 TCATGA T^CATGA
SfcI 44 CTATAG C^T[GA][CT]AG
StyI 1172 CCAAGG C^C[AT][AT]GG
TseI 1077 GCTGC G^C[AT]GC
TspRI 968 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcsI 950 GAATTT [GA]^AATT[CT]
1302 AAATTT
ApoI 950 GAATTT [GA]^AATT[CT]
1302 AAATTT
BalI 224 TGGCCA TGG^CCA
701 TGGCCA
BclI 740 TGATCA T^GATCA
1238 TGATCA
BmyI 373 GTGCTC G[AGT]GC[ACT]^C
642 GTGCCC
BsaI 516 GAGACC GGTCTC(1/5)
1018 GGTCTC
BsaJI 645 CCCAGG C^C[ACGT][ACGT]GG
1172 CCAAGG
Bsp1286I 373 GTGCTC G[AGT]GC[ACT]^C
642 GTGCCC
BsrI 526 CCAGT ACTGG(1/-1)
850 CCAGT
BssKI 399 CCAGG ^CC[ACGT]GG
646 CCAGG
BstNI 399 CCAGG CC^[AT]GG
646 CCAGG
BstYI 195 AGATCC [GA]^GATC[CT]
233 AGATCT
Cac8I 612 GCAAGC GC[ACGT]^[ACGT]GC
895 GCAAGC
EaeI 224 TGGCCA [CT]^GGCC[GA]
701 TGGCCA
EarI 1082 CTCTTC CTCTTC(1/4)
513 GAAGAG
EcoRII 399 CCAGG ^CC[AT]GG
646 CCAGG
FbaI 740 TGATCA T^GATCA
1238 TGATCA
HindIII 614 AAGCTT A^AGCTT
897 AAGCTT
Ksp632I 1082 CTCTTC CTCTTC(1/4)
513 GAAGAG
MflI 195 AGATCC [GA]^GATC[CT]
233 AGATCT
MluNI 224 TGGCCA TGG^CCA
701 TGGCCA
MscI 224 TGGCCA TGG^CCA
701 TGGCCA
MspA1I 493 CAGCTG C[AC]G^C[GT]G
1153 CAGCTG
MvaI 399 CCAGG CC^[AT]GG
646 CCAGG
NlaIII 744 CATG CATG^
1212 CATG
NlaIV 402 GGCACC GG[ACGT]^[ACGT]CC
1194 GGCTCC
PvuII 493 CAGCTG CAG^CTG
1153 CAGCTG
ScrFI 399 CCAGG CC^[ACGT]GG
646 CCAGG
TfiI 316 GATTC G^A[AT]TC
1008 GATTC
XbaI 681 TCTAGA T^CTAGA
999 TCTAGA
XhoII 195 AGATCC [GA]^GATC[CT]
233 AGATCT
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI BfaI BsiYI BslI
BsmAI BstF5I DdeI DpnII FokI
HaeIII HinfI HphI MaeI MaeIII
MboI MboII MnlI MseI MslI
MwoI NdeII RsaI Sau3AI SfaNI
Tru9I Tsp45I Tsp509I