Restriction map for
pFN21AE6245
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BseRI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HphI
KpnI Ksp632I MaeI MamI MboI
MboII MfeI MflI MluI MluNI
MroI MscI MunI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 56 enzymes) #
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AfaI 507 GTAC GT^AC
Asp700I 417 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 253 GAGCTC G[AT]GC[AT]^C
AvaI 626 CCCGGG C^[CT]CG[GA]G
BbrPI 763 CACGTG CAC^GTG
BglI 264 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 723 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 723 GCTGAGC GC^T[ACGT]AGC
BsaAI 763 CACGTG [CT]AC^GT[GA]
BsaI 631 GAGACC GGTCTC(1/5)
BsgI 512 GTGCAG GTGCAG(16/14)
BsiEI 57 CGGCCG CG[GA][CT]^CG
BsiHKAI 253 GAGCTC G[AT]GC[AT]^C
BsmAI 631 GAGAC GTCTC(1/5)
BsmFI 468 GTCCC GGGAC(10/14)
BsoBI 626 CCCGGG C^[CT]CG[GA]G
BsrFI 220 GCCGGC [GA]^CCGG[CT]
BssSI 527 CTCGTG CACGAG(-5/-1)
BstF5I 315 GGATG GGATG(2/0)
CelII 723 GCTGAGC GC^T[ACGT]AGC
Cfr10I 220 GCCGGC [GA]^CCGG[CT]
EaeI 57 CGGCCG [CT]^GGCC[GA]
EagI 57 CGGCCG C^GGCCG
EclXI 57 CGGCCG C^GGCCG
Eco52I 57 CGGCCG C^GGCCG
EcoNI 487 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 266 CCAAGG C^C[AT][AT]GG
FokI 315 GGATG GGATG(9/13)
HincII 457 GTTAAC GT[CT]^[GA]AC
HindII 457 GTTAAC GT[CT]^[GA]AC
HinfI 346 GACTC G^A[ACGT]TC
HpaI 457 GTTAAC GTT^AAC
KspI 48 CCGCGG CCGC^GG
MaeII 764 ACGT A^CGT
MaeIII 344 GTGAC ^GT[ACGT]AC
MseI 458 TTAA T^TAA
NaeI 220 GCCGGC GCC^GGC
NgoMIV 220 GCCGGC G^CCGGC
PflMI 543 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 346 GACTC GAGTC(4/5)
PmaCI 763 CACGTG CAC^GTG
PmlI 763 CACGTG CAC^GTG
RsaI 507 GTAC GT^AC
SacI 253 GAGCTC GAGCT^C
SacII 48 CCGCGG CCGC^GG
SmaI 626 CCCGGG CCC^GGG
SmlI 353 CTCAAG C^T[CT][GA]AG
StuI 419 AGGCCT AGG^CCT
StyI 266 CCAAGG C^C[AT][AT]GG
Tru9I 458 TTAA T^TAA
Tsp45I 344 GTGAC ^GT[GC]AC
Van91I 543 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 323 CCAAAGGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 626 CCCGGG C^CCGGG
XmaI 626 CCCGGG C^CCGGG
XmnI 417 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BsrBI 73 CCGCTC CCGCTC(-3/-3)
169 GAGCGG
BsrI 368 ACTGG ACTGG(1/-1)
732 ACTGG
BstAPI 228 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
276 GCAGCGACTGC
HgaI 63 GACGC GACGC(5/10)
410 GCGTC
MslI 351 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
606 CACAGACATG
NspI 611 ACATGC [GA]CATG^[CT]
650 ACATGC
PpuMI 545 AGGTCCT [GA]G^G[AT]CC[CT]
594 AGGTCCT
PstI 274 CTGCAG CTGCA^G
664 CTGCAG
PvuII 145 CAGCTG CAG^CTG
232 CAGCTG
SfcI 274 CTGCAG C^T[GA][CT]AG
664 CTGCAG
TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^
374 CACTG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
Sau96I ScrFI SfoI TseI