Restriction map for
pFN21AE2561
##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI Aor51HI AscI AspEI AviII
AvrII BamHI BbeI BfrI BlnI
BseAI BsiEI BsiWI BspEI BspHI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FseI FspI
HpaI KasI MluI MroI NarI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SbfI SfoI SfuI
SgrAI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 4979 GGTACC G^GTACC
AseI 7501 ATTAAT AT^TAAT
AsnI 7501 ATTAAT AT^TAAT
Asp718I 4979 GGTACC G^GTACC
BclI 5931 TGATCA T^GATCA
BspDI 1988 ATCGAT AT^CGAT
ClaI 1988 ATCGAT AT^CGAT
DraI 7335 TTTAAA TTT^AAA
EcoRI 5216 GAATTC G^AATTC
EcoRV 5125 GATATC GAT^ATC
FbaI 5931 TGATCA T^GATCA
HaeII 4327 AGCGCC [GA]GCGC^[CT]
HgaI 6550 GACGC GACGC(5/10)
HincII 6562 GTCGAC GT[CT]^[GA]AC
HindII 6562 GTCGAC GT[CT]^[GA]AC
KpnI 4979 GGTACC GGTAC^C
NaeI 4012 GCCGGC GCC^GGC
NdeI 3187 CATATG CA^TATG
NgoMIV 4012 GCCGGC G^CCGGC
PaeR7I 7016 CTCGAG C^TCGAG
PshBI 7501 ATTAAT AT^TAAT
SacI 5596 GAGCTC GAGCT^C
SalI 6562 GTCGAC G^TCGAC
SexAI 7255 ACCAGGT A^CC[AT]GGT
SfiI 307 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 756 TACGTA TAC^GTA
SphI 3650 GCATGC GCATG^C
SspI 3163 AATATT AAT^ATT
XhoI 7016 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3392 GACGTC GACGT^C
3589 GACGTC
AccII 4786 CGCG CG^CG
7060 CGCG
Alw44I 4491 GTGCAC G^TGCAC
4587 GTGCAC
ApaI 315 GGGCCC GGGCC^C
2491 GGGCCC
ApaLI 4491 GTGCAC G^TGCAC
4587 GTGCAC
Asp700I 1245 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3138 GAAAAGCTTC
BbrPI 4494 CACGTG CAC^GTG
6195 CACGTG
BbvCI 2480 CCTCAGC CCTCAGC(-5/-2)
7568 CCTCAGC
BciVI 6337 GGATAC GTATCC(6/5)
6579 GGATAC
BlpI 6731 GCTCAGC GC^T[ACGT]AGC
7010 GCTCAGC
Bpu1102I 6731 GCTCAGC GC^T[ACGT]AGC
7010 GCTCAGC
BsmBI 5073 CGTCTC CGTCTC(1/5)
2444 GAGACG
Bsp1407I 42 TGTACA T^GTACA
784 TGTACA
BspLU11I 1355 ACATGT A^CATGT
3242 ACATGT
BsrFI 4012 GCCGGC [GA]^CCGG[CT]
5968 ACCGGC
BsrGI 42 TGTACA T^GTACA
784 TGTACA
BstEII 350 GGTCACC G^GT[ACGT]ACC
5551 GGTGACC
BstPI 350 GGTCACC G^GT[ACGT]ACC
5551 GGTGACC
BstUI 4786 CGCG CG^CG
7060 CGCG
BstXI 6786 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7400 CCATCATCCTGG
CelII 6731 GCTCAGC GC^T[ACGT]AGC
7010 GCTCAGC
CfoI 137 GCGC GCG^C
4328 GCGC
Cfr10I 4012 GCCGGC [GA]^CCGG[CT]
5968 ACCGGC
DrdI 1371 GACTGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7044 GACTGACCTGTC
EcoO65I 350 GGTCACC G^GT[ACGT]ACC
5551 GGTGACC
HhaI 137 GCGC GCG^C
4328 GCGC
HinP1I 137 GCGC G^CGC
4328 GCGC
KspI 4785 CCGCGG CCGC^GG
7059 CCGCGG
MfeI 3305 CAATTG C^AATTG
3494 CAATTG
MunI 3305 CAATTG C^AATTG
3494 CAATTG
MvnI 4786 CGCG CG^CG
7060 CGCG
PflMI 3774 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5486 CCACTGCCTGG
PmaCI 4494 CACGTG CAC^GTG
6195 CACGTG
PmlI 4494 CACGTG CAC^GTG
6195 CACGTG
SacII 4785 CCGCGG CCGC^GG
7059 CCGCGG
SapI 2133 GAAGAGC GCTCTTC(1/4)
7271 GAAGAGC
ScaI 5134 AGTACT AGT^ACT
5936 AGTACT
SmaI 2797 CCCGGG CCC^GGG
6435 CCCGGG
SspBI 42 TGTACA T^GTACA
784 TGTACA
Van91I 3774 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5486 CCACTGCCTGG
XbaI 3579 TCTAGA T^CTAGA
7032 TCTAGA
XmaCI 2797 CCCGGG C^CCGGG
6435 CCCGGG
XmaI 2797 CCCGGG C^CCGGG
6435 CCCGGG
XmnI 1245 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3138 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII Hin1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII