Restriction map for
pFN21AE2123
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BbrPI BcgI BciVI BfrI
BlnI BsiWI BsmI BspDI BspHI
BsrDI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI DraI DrdI Eam1105I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacII SalI SapI SbfI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 2435 ACATGT A^C[GA][CT]GT
Alw44I 2125 GTGCAC G^TGCAC
ApaLI 2125 GTGCAC G^TGCAC
Asp700I 1788 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2629 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 242 TGCGCA TGC^GCA
BclI 212 TGATCA T^GATCA
BsaAI 679 TACGTG [CT]AC^GT[GA]
BsaBI 2163 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2242 GAGACC GGTCTC(1/5)
BsmBI 1666 GAGACG CGTCTC(1/5)
Bsp1407I 2438 TGTACA T^GTACA
BspLU11I 2435 ACATGT A^CATGT
BsrGI 2438 TGTACA T^GTACA
BstAPI 378 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2454 GGTGACC G^GT[ACGT]ACC
BstPI 2454 GGTGACC G^GT[ACGT]ACC
CpoI 340 CGGACCG CG^G[AT]CCG
DraIII 379 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1779 GAAGAG CTCTTC(1/4)
EcoO65I 2454 GGTGACC G^GT[ACGT]ACC
FbaI 212 TGATCA T^GATCA
FspI 242 TGCGCA TGC^GCA
HincII 638 GTCAAC GT[CT]^[GA]AC
HindII 638 GTCAAC GT[CT]^[GA]AC
Ksp632I 1779 GAAGAG CTCTTC(1/4)
MamI 2163 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 578 TTAA T^TAA
NcoI 2495 CCATGG C^CATGG
NheI 822 GCTAGC G^CTAGC
PflFI 2629 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT]
RsrII 340 CGGACCG CG^G[AT]CCG
ScaI 620 AGTACT AGT^ACT
SfiI 18 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2438 TGTACA T^GTACA
Tru9I 578 TTAA T^TAA
Tsp509I 1865 AATT ^AATT
Tth111I 2629 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1788 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 772 CCTGAGG CC^T[ACGT]AGG
1965 CCTCAGG
BamHI 1023 GGATCC G^GATCC
1421 GGATCC
BbeI 43 GGCGCC GGCGC^C
1278 GGCGCC
BbvCI 2091 CCTCAGC CCTCAGC(-5/-2)
2285 CCTCAGC
BglII 1161 AGATCT A^GATCT
1550 AGATCT
BsrBI 2368 CCGCTC CCGCTC(-3/-3)
1891 GAGCGG
BsrFI 858 GCCGGC [GA]^CCGG[CT]
2134 GCCGGC
BstXI 1379 CCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2297 CCACGCCCGTGG
Bsu36I 772 CCTGAGG CC^T[ACGT]AGG
1965 CCTCAGG
Cfr10I 858 GCCGGC [GA]^CCGG[CT]
2134 GCCGGC
Eco81I 772 CCTGAGG CC^T[ACGT]AGG
1965 CCTCAGG
EcoNI 240 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2511 CCTGGAGGAGG
FauI 1251 GCGGG CCCGC(4/6)
2099 GCGGG
KasI 43 GGCGCC G^GCGCC
1278 GGCGCC
NaeI 858 GCCGGC GCC^GGC
2134 GCCGGC
NarI 43 GGCGCC GG^CGCC
1278 GGCGCC
NgoMIV 858 GCCGGC G^CCGGC
2134 GCCGGC
PaeR7I 817 CTCGAG C^TCGAG
839 CTCGAG
PflMI 1224 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2379 CCAGCAGGTGG
SexAI 380 ACCTGGT A^CC[AT]GGT
1718 ACCTGGT
SfoI 43 GGCGCC GGC^GCC
1278 GGCGCC
Van91I 1224 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2379 CCAGCAGGTGG
XbaI 520 TCTAGA T^CTAGA
1044 TCTAGA
XcmI 2070 CCAGCTCAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2294 CCACCACGCCCGTGG
XhoI 817 CTCGAG C^TCGAG
839 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI Aor51HI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco47III Eco52I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XhoII