##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     Tth111I     
TthHB8I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        272  GGTACC               G^GTACC
AccII          14  CGCG                 CG^CG
AocI          381  CCTCAGG              CC^T[ACGT]AGG
Asp718I       272  GGTACC               G^GTACC
BanI          272  GGTACC               G^G[CT][GA]CC
BcgI          294  CGAGGGCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsgI          132  CTGCAC               GTGCAG(16/14)
BsrBI         268  GAGCGG               CCGCTC(-3/-3)
BstF5I         93  GGATG                GGATG(2/0)
BstUI          14  CGCG                 CG^CG
Bsu36I        381  CCTCAGG              CC^T[ACGT]AGG
DraII         242  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          182  CCGTGG               C^C[GA][CT]GG
EaeI          290  TGGCCG               [CT]^GGCC[GA]
Eco81I        381  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      242  GGGTCCT              [GA]G^G[ACGT]CC[CT]
FauI          143  CCCGC                CCCGC(4/6)
Fnu4HI        375  GCGGC                GC^[ACGT]GC
FokI           93  GGATG                GGATG(9/13)
HapII         357  CCGG                 C^CGG
HinfI         121  GAGTC                G^A[ACGT]TC
HpaII         357  CCGG                 C^CGG
HphI          284  TCACC                GGTGA(8/7)
ItaI          375  GCGGC                GC^[ACGT]GC
KpnI          272  GGTACC               GGTAC^C
MspI          357  CCGG                 C^CGG
MvnI           14  CGCG                 CG^CG
MwoI          339  GCTCGCCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI         312  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          121  GAGTC                GAGTC(4/5)
PpuMI         242  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         237  GACCAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Van91I        312  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          278  CCATGTTCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BbvCI         246  CCTCAGC              CCTCAGC(-5/-2)
              257  CCTCAGC             
BpmI          118  CTGGAG               CTGGAG(16/14)
              197  CTGGAG              
BsmAI         200  GAGAC                GTCTC(1/5)
              217  GAGAC               
Cac8I          80  GCCAGC               GC[ACGT]^[ACGT]GC
              339  GCTCGC              
EcoNI         288  CCTGGCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              364  CCTCGCACAGG         
NlaIII        279  CATG                 CATG^
              301  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BsaJI       
BseRI       BsiYI       BslI        BssKI       BstNI       
Cfr13I      DdeI        EcoRII      HaeIII      MnlI        
MvaI        NlaIV       RsaI        Sau96I      ScrFI       
TspRI