Restriction map for
pFN21AE6095
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaJI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 912 GCGG CCGC(-3/-1)
AcsI 94 GAATTT [GA]^AATT[CT]
AflIII 754 ACATGT A^C[GA][CT]GT
ApoI 94 GAATTT [GA]^AATT[CT]
AseI 75 ATTAAT AT^TAAT
AsnI 75 ATTAAT AT^TAAT
Asp700I 367 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 761 GAGCTC G[AT]GC[AT]^C
BalI 704 TGGCCA TGG^CCA
BamHI 544 GGATCC G^GATCC
BbvI 382 GCTGC GCAGC(8/12)
BpmI 773 CTGGAG CTGGAG(16/14)
BsaAI 613 CACGTA [CT]AC^GT[GA]
BsaBI 19 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 816 GAGACC GGTCTC(1/5)
BsiHKAI 761 GAGCTC G[AT]GC[AT]^C
BsiYI 178 CCGATCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 178 CCGATCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 816 GAGAC GTCTC(1/5)
BsmFI 793 GGGAC GGGAC(10/14)
BsmI 367 GAATGC GAATGC(1/-1)
BspLU11I 754 ACATGT A^CATGT
BsrDI 385 GCAATG GCAATG(2/0)
BsrI 772 ACTGG ACTGG(1/-1)
BssKI 291 CCTGG ^CC[ACGT]GG
BstNI 291 CCTGG CC^[AT]GG
Cac8I 487 GCTGGC GC[ACGT]^[ACGT]GC
EaeI 704 TGGCCA [CT]^GGCC[GA]
EarI 802 CTCTTC CTCTTC(1/4)
EcoRII 291 CCTGG ^CC[AT]GG
Fnu4HI 382 GCTGC GC^[ACGT]GC
HaeIII 705 GGCC GG^CC
ItaI 382 GCTGC GC^[ACGT]GC
Ksp632I 802 CTCTTC CTCTTC(1/4)
MaeII 614 ACGT A^CGT
MamI 19 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 704 TGGCCA TGG^CCA
MscI 704 TGGCCA TGG^CCA
MvaI 291 CCTGG CC^[AT]GG
MwoI 382 GCTGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 452 GAGTC GAGTC(4/5)
PshBI 75 ATTAAT AT^TAAT
SacI 761 GAGCTC GAGCT^C
ScrFI 291 CCTGG CC^[ACGT]GG
SfaNI 717 GATGC GCATC(5/9)
SspI 861 AATATT AAT^ATT
TseI 382 GCTGC G^C[AT]GC
XmnI 367 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BanII 645 GGGCTC G[GA]GC[CT]^C
761 GAGCTC
BbsI 109 GTCTTC GAAGAC(2/6)
745 GTCTTC
BmyI 645 GGGCTC G[AGT]GC[ACT]^C
761 GAGCTC
BpuAI 109 GTCTTC GAAGAC(2/6)
745 GTCTTC
BseRI 581 CTCCTC GAGGAG(10/8)
735 GAGGAG
Bsp1286I 645 GGGCTC G[AGT]GC[ACT]^C
761 GAGCTC
BstF5I 243 GGATG GGATG(2/0)
322 GGATG
BstYI 24 GGATCT [GA]^GATC[CT]
544 GGATCC
FokI 243 GGATG GGATG(9/13)
322 GGATG
HindIII 34 AAGCTT A^AGCTT
81 AAGCTT
MflI 24 GGATCT [GA]^GATC[CT]
544 GGATCC
MseI 43 TTAA T^TAA
76 TTAA
MslI 807 CATGTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
849 CATGATAGTG
NlaIV 358 GGAACC GG[ACGT]^[ACGT]CC
544 GGATCC
SfcI 257 CTACAG C^T[GA][CT]AG
903 CTACAG
TfiI 879 GATTC G^A[AT]TC
891 GAATC
Tru9I 43 TTAA T^TAA
76 TTAA
XhoII 24 GGATCT [GA]^GATC[CT]
544 GGATCC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
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AfaI AluI AlwI DpnII HinfI
HphI MaeIII MboI MboII MnlI
NdeII NlaIII NspI RsaI Sau3AI
Tsp45I Tsp509I TspRI