Restriction map for
pFN21AE6065
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BcnI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI Fnu4HI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII ItaI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MroI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TaqI TseI
Tsp45I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 568 GTAGAC GT^[AC][GT]AC
AciI 859 CCGC CCGC(-3/-1)
AflII 873 CTTAAG C^TTAAG
AlwNI 238 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 458 TGGCCA TGG^CCA
BanII 321 GGGCTC G[GA]GC[CT]^C
BfaI 205 CTAG C^TAG
BfrI 873 CTTAAG C^TTAAG
BglII 207 AGATCT A^GATCT
BmyI 321 GGGCTC G[AGT]GC[ACT]^C
BpmI 444 CTGGAG CTGGAG(16/14)
BsaBI 429 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 807 CCAGGG C^C[ACGT][ACGT]GG
BsmAI 851 GTCTC GTCTC(1/5)
BsmFI 258 GGGAC GGGAC(10/14)
Bsp1286I 321 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 478 TGTACA T^GTACA
BspMI 776 ACCTGC ACCTGC(4/8)
BsrBI 859 CCGCTC CCGCTC(-3/-3)
BsrGI 478 TGTACA T^GTACA
DraII 258 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 458 TGGCCA [CT]^GGCC[GA]
EcoO109I 258 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 858 CCCGC CCCGC(4/6)
HaeIII 459 GGCC GG^CC
HincII 247 GTCAAC GT[CT]^[GA]AC
HindII 247 GTCAAC GT[CT]^[GA]AC
HinfI 154 GATTC G^A[ACGT]TC
MaeI 205 CTAG C^TAG
MamI 429 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 458 TGGCCA TGG^CCA
MscI 458 TGGCCA TGG^CCA
MslI 413 CACATCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 258 GGGACC GG[ACGT]^[ACGT]CC
PpuMI 258 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 783 ACCAGGT A^CC[AT]GGT
SmlI 873 CTTAAG C^T[CT][GA]AG
SspBI 478 TGTACA T^GTACA
TfiI 154 GATTC G^A[AT]TC
TspRI 834 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 272 CCATCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 192 AAATTT [GA]^AATT[CT]
492 GAATTT
ApoI 192 AAATTT [GA]^AATT[CT]
492 GAATTT
AvaII 3 GGACC G^G[AT]CC
259 GGACC
BsiYI 336 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCCACATGTGG
BslI 336 CCTTCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCCACATGTGG
BspHI 404 TCATGA T^CATGA
437 TCATGA
BsrDI 660 CATTGC GCAATG(2/0)
709 GCAATG
BstYI 207 AGATCT [GA]^GATC[CT]
724 GGATCT
Cfr13I 3 GGACC G^G[ACGT]CC
259 GGACC
HphI 821 TCACC GGTGA(8/7)
921 TCACC
MaeIII 122 GTAAC ^GT[ACGT]AC
788 GTAAC
MboII 903 GAAGA GAAGA(8/7)
98 TCTTC
MflI 207 AGATCT [GA]^GATC[CT]
724 GGATCT
MseI 106 TTAA T^TAA
874 TTAA
RcaI 404 TCATGA T^CATGA
437 TCATGA
Sau96I 3 GGACC G^G[ACGT]CC
259 GGACC
SfcI 467 CTGTAG C^T[GA][CT]AG
737 CTGTAG
Tru9I 106 TTAA T^TAA
874 TTAA
XhoII 207 AGATCT [GA]^GATC[CT]
724 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AflIII AluI AlwI BspLU11I
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII FokI MboI MnlI
MvaI NdeII NlaIII NspI RsaI
Sau3AI ScrFI SfaNI Tsp509I