Restriction map for
pFN21AE6044
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI Ksp632I
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 656 TCCGGA T^CCGGA
AvaII 793 GGTCC G^G[AT]CC
BalI 431 TGGCCA TGG^CCA
BbrPI 717 CACGTG CAC^GTG
BbsI 826 GTCTTC GAAGAC(2/6)
BpuAI 826 GTCTTC GAAGAC(2/6)
BsaAI 717 CACGTG [CT]AC^GT[GA]
BsaWI 656 TCCGGA [AT]^CCGG[AT]
BseAI 656 TCCGGA T^CCGGA
BseRI 576 CTCCTC GAGGAG(10/8)
BspEI 656 TCCGGA T^CCGGA
BspHI 107 TCATGA T^CATGA
BstYI 680 AGATCC [GA]^GATC[CT]
DraII 895 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 895 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 72 GATATC GAT^ATC
EcoT22I 406 ATGCAT ATGCA^T
HapII 657 CCGG C^CGG
HincII 930 GTTAAC GT[CT]^[GA]AC
HindII 930 GTTAAC GT[CT]^[GA]AC
HinfI 161 GACTC G^A[ACGT]TC
HpaI 930 GTTAAC GTT^AAC
HpaII 657 CCGG C^CGG
MflI 680 AGATCC [GA]^GATC[CT]
MluNI 431 TGGCCA TGG^CCA
MroI 656 TCCGGA T^CCGGA
MscI 431 TGGCCA TGG^CCA
MseI 931 TTAA T^TAA
MspI 657 CCGG C^CGG
NsiI 406 ATGCAT ATGCA^T
PleI 161 GACTC GAGTC(4/5)
PmaCI 717 CACGTG CAC^GTG
PmlI 717 CACGTG CAC^GTG
RcaI 107 TCATGA T^CATGA
TaqI 206 TCGA T^CGA
Tru9I 931 TTAA T^TAA
TthHB8I 206 TCGA T^CGA
XcmI 780 CCAGCCCCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 680 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
384 CCGC
AcsI 31 GAATTT [GA]^AATT[CT]
919 AAATTC
AflIII 716 ACACGT A^C[GA][CT]GT
718 ACGTGT
Alw44I 63 GTGCAC G^TGCAC
504 GTGCAC
AlwI 681 GATCC GGATC(4/5)
659 GGATC
AlwNI 54 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
238 CAGCTGCTG
ApaLI 63 GTGCAC G^TGCAC
504 GTGCAC
ApoI 31 GAATTT [GA]^AATT[CT]
919 AAATTC
Asp700I 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
705 GAAAGCCTTC
AspHI 63 GTGCAC G[AT]GC[AT]^C
504 GTGCAC
BclI 143 TGATCA T^GATCA
522 TGATCA
BmyI 63 GTGCAC G[AGT]GC[ACT]^C
504 GTGCAC
BsgI 290 GTGCAG GTGCAG(16/14)
472 GTGCAG
BsiHKAI 63 GTGCAC G[AT]GC[AT]^C
504 GTGCAC
BsmAI 128 GTCTC GTCTC(1/5)
211 GTCTC
BsmFI 232 GTCCC GGGAC(10/14)
794 GTCCC
Bsp1286I 63 GTGCAC G[AGT]GC[ACT]^C
504 GTGCAC
Cfr13I 793 GGTCC G^G[ACGT]CC
896 GGGCC
EaeI 398 CGGCCA [CT]^GGCC[GA]
431 TGGCCA
EcoT14I 42 CCTTGG C^C[AT][AT]GG
317 CCTTGG
FbaI 143 TGATCA T^GATCA
522 TGATCA
MaeII 19 ACGT A^CGT
718 ACGT
MaeIII 56 GTGAC ^GT[ACGT]AC
97 GTCAC
MspA1I 238 CAGCTG C[AC]G^C[GT]G
493 CAGCTG
PflMI 53 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
237 CCAGCTGCTGG
PvuII 238 CAGCTG CAG^CTG
493 CAGCTG
Sau96I 793 GGTCC G^G[ACGT]CC
896 GGGCC
StyI 42 CCTTGG C^C[AT][AT]GG
317 CCTTGG
Tsp45I 56 GTGAC ^GT[GC]AC
97 GTCAC
Tsp509I 32 AATT ^AATT
920 AATT
Van91I 53 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
237 CCAGCTGCTGG
XmnI 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
705 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeI
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
ScrFI TseI TspRI