Restriction map for
pFN21AE5972
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 867 CTTAAG C^TTAAG
AlwI 316 GGATC GGATC(4/5)
AlwNI 432 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 220 GTCTTC GAAGAC(2/6)
BbvI 546 GCTGC GCAGC(8/12)
BfrI 867 CTTAAG C^TTAAG
BpmI 504 CTCCAG CTGGAG(16/14)
BpuAI 220 GTCTTC GAAGAC(2/6)
BsmAI 257 GAGAC GTCTC(1/5)
BspHI 398 TCATGA T^CATGA
BspMI 879 GCAGGT ACCTGC(4/8)
BsrDI 246 CATTGC GCAATG(2/0)
BsrI 489 ACTGG ACTGG(1/-1)
BssKI 437 CCTGG ^CC[ACGT]GG
BstF5I 654 CATCC GGATG(2/0)
BstNI 437 CCTGG CC^[AT]GG
DraII 890 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 284 CCATGG C^C[GA][CT]GG
EcoO109I 890 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 437 CCTGG ^CC[AT]GG
Fnu4HI 546 GCTGC GC^[ACGT]GC
FokI 654 CATCC GGATG(9/13)
HincII 135 GTTAAC GT[CT]^[GA]AC
HindII 135 GTTAAC GT[CT]^[GA]AC
HpaI 135 GTTAAC GTT^AAC
ItaI 546 GCTGC GC^[ACGT]GC
MvaI 437 CCTGG CC^[AT]GG
MwoI 280 GCTTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 284 CCATGG C^CATGG
NdeI 724 CATATG CA^TATG
NheI 597 GCTAGC G^CTAGC
PleI 502 GACTC GAGTC(4/5)
RcaI 398 TCATGA T^CATGA
ScrFI 437 CCTGG CC^[ACGT]GG
SfcI 251 CTACAG C^T[GA][CT]AG
SmlI 867 CTTAAG C^T[CT][GA]AG
TseI 546 GCTGC G^C[AT]GC
Tsp45I 500 GTGAC ^GT[GC]AC
TspRI 818 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 576 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 433 AGCT AG^CT
581 AGCT
AocI 180 CCTCAGG CC^T[ACGT]AGG
894 CCTTAGG
Asp700I 25 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
325 GAATTTTTTC
BsaJI 284 CCATGG C^C[ACGT][ACGT]GG
629 CCTTGG
BsiYI 483 CCTCCAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
686 CCCAGCAAAGG
BslI 483 CCTCCAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
686 CCCAGCAAAGG
Bsu36I 180 CCTCAGG CC^T[ACGT]AGG
894 CCTTAGG
Eco81I 180 CCTCAGG CC^T[ACGT]AGG
894 CCTTAGG
EcoRI 25 GAATTC G^AATTC
901 GAATTC
EcoT14I 284 CCATGG C^C[AT][AT]GG
629 CCTTGG
HphI 882 GGTGA GGTGA(8/7)
905 TCACC
SspI 148 AATATT AAT^ATT
915 AATATT
StyI 284 CCATGG C^C[AT][AT]GG
629 CCTTGG
XmnI 25 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
325 GAATTTTTTC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AcsI ApoI BclI BfaI Cac8I
Cfr13I DdeI DpnII FbaI HaeIII
HinfI MaeI MaeIII MboI MboII
MnlI MseI MslI NdeII NlaIII
Sau3AI Sau96I TfiI Tru9I Tsp509I