Restriction map for
pFN21AE5915
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FbaI
FseI HapII HgaI HpaI HpaII
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MspA1I MspI
MunI MwoI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 41 CGCG CG^CG
AcyI 889 GGCGCC G[GA]^CG[CT]C
Alw44I 417 GTGCAC G^TGCAC
ApaLI 417 GTGCAC G^TGCAC
AspHI 417 GTGCAC G[AT]GC[AT]^C
AviII 650 TGCGCA TGC^GCA
BalI 194 TGGCCA TGG^CCA
BamHI 674 GGATCC G^GATCC
BanI 889 GGCGCC G^G[CT][GA]CC
BbeI 889 GGCGCC GGCGC^C
BsaHI 889 GGCGCC G[GA]^CG[CT]C
BsaI 639 GGTCTC GGTCTC(1/5)
BsaJI 440 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 417 GTGCAC G[AT]GC[AT]^C
BsmAI 640 GTCTC GTCTC(1/5)
BsmI 873 GAATGC GAATGC(1/-1)
BspMI 712 ACCTGC ACCTGC(4/8)
BstUI 41 CGCG CG^CG
BstYI 674 GGATCC [GA]^GATC[CT]
EarI 742 CTCTTC CTCTTC(1/4)
EcoT22I 285 ATGCAT ATGCA^T
FspI 650 TGCGCA TGC^GCA
Hin1I 889 GGCGCC G[GA]^CG[CT]C
HincII 202 GTTGAC GT[CT]^[GA]AC
HindII 202 GTTGAC GT[CT]^[GA]AC
HindIII 157 AAGCTT A^AGCTT
KasI 889 GGCGCC G^GCGCC
Ksp632I 742 CTCTTC CTCTTC(1/4)
MflI 674 GGATCC [GA]^GATC[CT]
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
MvnI 41 CGCG CG^CG
NarI 889 GGCGCC GG^CGCC
NsiI 285 ATGCAT ATGCA^T
PleI 740 GACTC GAGTC(4/5)
SexAI 578 ACCAGGT A^CC[AT]GGT
SfoI 889 GGCGCC GGC^GCC
StuI 701 AGGCCT AGG^CCT
Tsp45I 763 GTCAC ^GT[GC]AC
XhoII 674 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 174 GTAC GT^AC
768 GTAC
AvaII 55 GGTCC G^G[AT]CC
468 GGTCC
BmyI 417 GTGCAC G[AGT]GC[ACT]^C
902 GGGCAC
BpmI 64 CTGGAG CTGGAG(16/14)
408 CTGGAG
BsaAI 322 CACGTA [CT]AC^GT[GA]
765 CACGTA
Bsp1286I 417 GTGCAC G[AGT]GC[ACT]^C
902 GGGCAC
BspHI 401 TCATGA T^CATGA
510 TCATGA
BsrI 63 ACTGG ACTGG(1/-1)
678 CCAGT
Cac8I 503 GCGGGC GC[ACGT]^[ACGT]GC
598 GCCTGC
EaeI 194 TGGCCA [CT]^GGCC[GA]
659 TGGCCG
FauI 785 CCCGC CCCGC(4/6)
503 GCGGG
HaeII 754 AGCGCC [GA]GCGC^[CT]
889 GGCGCC
MaeIII 389 GTTAC ^GT[ACGT]AC
763 GTCAC
MboII 179 TCTTC GAAGA(8/7)
743 TCTTC
RcaI 401 TCATGA T^CATGA
510 TCATGA
RsaI 174 GTAC GT^AC
768 GTAC
SfaNI 237 GATGC GCATC(5/9)
653 GCATC
SmlI 547 CTCAAG C^T[CT][GA]AG
669 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwI AocI BbvI
BseRI BsiYI BslI BsmFI BssKI
BstF5I BstNI Bsu36I CfoI Cfr13I
DdeI DpnII Eco81I EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MboI MnlI
MslI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TfiI TseI
TspRI