Restriction map for
pFN21AE5902
##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BbeI BbrPI BbvCI
BbvI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII ItaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI TseI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AciI 737 CCGC CCGC(-3/-1)
AlwNI 327 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 665 TGGCCA TGG^CCA
BamHI 360 GGATCC G^GATCC
BanI 52 GGTGCC G^G[CT][GA]CC
BanII 453 GGGCTC G[GA]GC[CT]^C
BbsI 684 GTCTTC GAAGAC(2/6)
BcgI 412 CGAACAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 74 GTATCC GTATCC(6/5)
BpuAI 684 GTCTTC GAAGAC(2/6)
BsaAI 174 CACGTA [CT]AC^GT[GA]
BsaJI 800 CCCAGG C^C[ACGT][ACGT]GG
BsgI 692 GTGCAG GTGCAG(16/14)
BsmAI 783 GTCTC GTCTC(1/5)
Bsp1407I 471 TGTACA T^GTACA
BsrGI 471 TGTACA T^GTACA
BstAPI 313 GCAACATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 303 CTTAG C^T[ACGT]AG
DraI 900 TTTAAA TTT^AAA
EaeI 665 TGGCCA [CT]^GGCC[GA]
EarI 79 CTCTTC CTCTTC(1/4)
EcoRI 31 GAATTC G^AATTC
EcoRV 208 GATATC GAT^ATC
HincII 574 GTTGAC GT[CT]^[GA]AC
HindII 574 GTTGAC GT[CT]^[GA]AC
HinfI 154 GATTC G^A[ACGT]TC
HphI 743 TCACC GGTGA(8/7)
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeII 175 ACGT A^CGT
MluNI 665 TGGCCA TGG^CCA
MscI 665 TGGCCA TGG^CCA
MslI 99 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PshAI 735 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 867 CTATAG C^T[GA][CT]AG
SmlI 873 CTTGAG C^T[CT][GA]AG
SspBI 471 TGTACA T^GTACA
TfiI 154 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 31 GAATTC [GA]^AATT[CT]
267 GAATTT
AfaI 177 GTAC GT^AC
472 GTAC
ApoI 31 GAATTC [GA]^AATT[CT]
267 GAATTT
BfaI 7 CTAG C^TAG
689 CTAG
BmyI 53 GTGCCC G[AGT]GC[ACT]^C
453 GGGCTC
BsmFI 757 GGGAC GGGAC(10/14)
408 GTCCC
Bsp1286I 53 GTGCCC G[AGT]GC[ACT]^C
453 GGGCTC
BssKI 342 CCTGG ^CC[ACGT]GG
801 CCAGG
BstF5I 423 CATCC GGATG(2/0)
672 CATCC
BstNI 342 CCTGG CC^[AT]GG
801 CCAGG
BstYI 360 GGATCC [GA]^GATC[CT]
610 GGATCT
DpnII 361 GATC ^GATC
611 GATC
EcoRII 342 CCTGG ^CC[AT]GG
801 CCAGG
FokI 423 CATCC GGATG(9/13)
672 CATCC
HaeIII 666 GGCC GG^CC
819 GGCC
HindIII 249 AAGCTT A^AGCTT
896 AAGCTT
MaeI 7 CTAG C^TAG
689 CTAG
MboI 361 GATC ^GATC
611 GATC
MflI 360 GGATCC [GA]^GATC[CT]
610 GGATCT
MvaI 342 CCTGG CC^[AT]GG
801 CCAGG
MwoI 313 GCAACATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
346 GCTATGATGGC
NdeII 361 GATC ^GATC
611 GATC
NlaIV 52 GGTGCC GG[ACGT]^[ACGT]CC
360 GGATCC
RsaI 177 GTAC GT^AC
472 GTAC
Sau3AI 361 GATC ^GATC
611 GATC
ScrFI 342 CCTGG CC^[ACGT]GG
801 CCAGG
XhoII 360 GGATCC [GA]^GATC[CT]
610 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AflIII AluI AlwI BspLU11I BsrI
MaeIII MboII MnlI MseI NlaIII
NspI SfaNI Tru9I Tsp45I Tsp509I
TspRI