Restriction map for
pFN21AE5766
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# NO CUT ENZYMES ( 133 enzymes) #
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AccII AccIII AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI BstXI BstYI
Bsu36I CelII CfoI Cfr10I CpoI
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FauI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KspI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 196 GACGTC GACGT^C
Acc65I 478 GGTACC G^GTACC
AccI 573 GTATAC GT^[AC][GT]AC
AcyI 196 GACGTC G[GA]^CG[CT]C
AfaI 479 GTAC GT^AC
AlwI 717 GGATC GGATC(4/5)
AlwNI 1214 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 504 GGGCCC GGGCC^C
Asp718I 478 GGTACC G^GTACC
AvaI 1633 CCCGGG C^[CT]CG[GA]G
BanI 478 GGTACC G^G[CT][GA]CC
BanII 504 GGGCCC G[GA]GC[CT]^C
BcgI 677 GCAATGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 657 GTATCC GTATCC(6/5)
BclI 1601 TGATCA T^GATCA
BsaHI 196 GACGTC G[GA]^CG[CT]C
BseRI 1734 CTCCTC GAGGAG(10/8)
BsgI 1643 GTGCAG GTGCAG(16/14)
BsiEI 848 CGGTCG CG[GA][CT]^CG
BsmAI 337 GAGAC GTCTC(1/5)
BsmFI 199 GTCCC GGGAC(10/14)
BsmI 760 GAATGC GAATGC(1/-1)
BsoBI 1633 CCCGGG C^[CT]CG[GA]G
BspDI 401 ATCGAT AT^CGAT
BsrDI 677 GCAATG GCAATG(2/0)
Bst1107I 573 GTATAC GTA^TAC
BstZ17I 573 GTATAC GTA^TAC
ClaI 401 ATCGAT AT^CGAT
DraI 1005 TTTAAA TTT^AAA
EcoT14I 566 CCAAGG C^C[AT][AT]GG
EcoT22I 1079 ATGCAT ATGCA^T
FbaI 1601 TGATCA T^GATCA
HapII 1634 CCGG C^CGG
Hin1I 196 GACGTC G[GA]^CG[CT]C
HindIII 1678 AAGCTT A^AGCTT
HpaII 1634 CCGG C^CGG
KpnI 478 GGTACC GGTAC^C
MaeIII 811 GTCAC ^GT[ACGT]AC
MspI 1634 CCGG C^CGG
NsiI 1079 ATGCAT ATGCA^T
NspI 1081 GCATGT [GA]CATG^[CT]
PleI 1137 GACTC GAGTC(4/5)
PpuMI 1685 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1331 CAGCTG CAG^CTG
RsaI 479 GTAC GT^AC
SapI 30 GCTCTTC GCTCTTC(1/4)
SfcI 1479 CTACAG C^T[GA][CT]AG
SmaI 1633 CCCGGG CCC^GGG
StyI 566 CCAAGG C^C[AT][AT]GG
Tsp45I 811 GTCAC ^GT[GC]AC
XmaCI 1633 CCCGGG C^CCGGG
XmaI 1633 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AhdI 248 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
493 GACACTTTGTC
AspEI 248 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
493 GACACTTTGTC
AspHI 461 GAGCAC G[AT]GC[AT]^C
1251 GTGCTC
AvaII 1686 GGTCC G^G[AT]CC
1756 GGACC
BcnI 1633 CCCGG CC^[GC]GG
1634 CCGGG
BsaBI 1027 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1044 GATTGAAATC
BsiHKAI 461 GAGCAC G[AT]GC[AT]^C
1251 GTGCTC
BstAPI 463 GCACTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1102 GCAGCTATTGC
BstF5I 1693 CATCC GGATG(2/0)
998 GGATG
DraII 1617 AGGGCCT [GA]G^G[ACGT]CC[CT]
1685 GGGTCCT
Eam1105I 248 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
493 GACACTTTGTC
EarI 31 CTCTTC CTCTTC(1/4)
334 GAAGAG
EcoO109I 1617 AGGGCCT [GA]G^G[ACGT]CC[CT]
1685 GGGTCCT
EcoRV 538 GATATC GAT^ATC
1288 GATATC
FokI 1693 CATCC GGATG(9/13)
998 GGATG
HgaI 528 GCGTC GACGC(5/10)
1670 GCGTC
Ksp632I 31 CTCTTC CTCTTC(1/4)
334 GAAGAG
MamI 1027 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1044 GATTGAAATC
MspA1I 1217 CCGCTG C[AC]G^C[GT]G
1331 CAGCTG
NciI 1633 CCCGG CC^[GC]GG
1634 CCGGG
PflMI 36 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
483 CCATGATTTGG
Van91I 36 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
483 CCATGATTTGG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeI MaeII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SspI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I