Restriction map for
pFN21AE5692
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AccII AclI AflII AflIII AgeI
AscI AseI AsnI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI EagI EarI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I XcmI
XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 456 GACGTC GACGT^C
AccI 649 GTCTAC GT^[AC][GT]AC
AccIII 146 TCCGGA T^CCGGA
AcsI 163 GAATTT [GA]^AATT[CT]
AcyI 456 GACGTC G[GA]^CG[CT]C
AhdI 433 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1168 GTGCAC G^TGCAC
AocI 1068 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1433 AGCGCT AGC^GCT
ApaI 878 GGGCCC GGGCC^C
ApaLI 1168 GTGCAC G^TGCAC
ApoI 163 GAATTT [GA]^AATT[CT]
Asp700I 907 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 433 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 668 CCCGGG C^[CT]CG[GA]G
BalI 129 TGGCCA TGG^CCA
BsaHI 456 GACGTC G[GA]^CG[CT]C
BsaWI 146 TCCGGA [AT]^CCGG[AT]
BseAI 146 TCCGGA T^CCGGA
BsiEI 1215 CGATCG CG[GA][CT]^CG
BsmFI 353 GGGAC GGGAC(10/14)
BsoBI 668 CCCGGG C^[CT]CG[GA]G
BspEI 146 TCCGGA T^CCGGA
BspMI 584 ACCTGC ACCTGC(4/8)
BsrDI 42 CATTGC GCAATG(2/0)
BstAPI 579 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1068 CCTCAGG CC^T[ACGT]AGG
EaeI 129 TGGCCA [CT]^GGCC[GA]
Eam1105I 433 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1433 AGCGCT AGC^GCT
Eco81I 1068 CCTCAGG CC^T[ACGT]AGG
FauI 1428 CCCGC CCCGC(4/6)
HaeII 1433 AGCGCT [GA]GCGC^[CT]
Hin1I 456 GACGTC G[GA]^CG[CT]C
HincII 1423 GTTGAC GT[CT]^[GA]AC
HindII 1423 GTTGAC GT[CT]^[GA]AC
MaeII 457 ACGT A^CGT
MaeIII 1372 GTCAC ^GT[ACGT]AC
MluNI 129 TGGCCA TGG^CCA
MroI 146 TCCGGA T^CCGGA
MscI 129 TGGCCA TGG^CCA
MseI 416 TTAA T^TAA
MslI 190 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 536 CAGCTG C[AC]G^C[GT]G
NspI 770 ACATGC [GA]CATG^[CT]
PvuI 1215 CGATCG CGAT^CG
PvuII 536 CAGCTG CAG^CTG
SacI 26 GAGCTC GAGCT^C
ScaI 224 AGTACT AGT^ACT
SmaI 668 CCCGGG CCC^GGG
SmlI 268 CTCAAG C^T[CT][GA]AG
Tru9I 416 TTAA T^TAA
Tsp45I 1372 GTCAC ^GT[GC]AC
Tsp509I 164 AATT ^AATT
XbaI 1017 TCTAGA T^CTAGA
XmaCI 668 CCCGGG C^CCGGG
XmaI 668 CCCGGG C^CCGGG
XmnI 907 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 112 GGTACC G^GTACC
798 GGTACC
AlwI 1275 GATCC GGATC(4/5)
411 GGATC
Asp718I 112 GGTACC G^GTACC
798 GGTACC
AspHI 26 GAGCTC G[AT]GC[AT]^C
1168 GTGCAC
BbsI 1084 GAAGAC GAAGAC(2/6)
1204 GTCTTC
BbvCI 803 CCTCAGC CCTCAGC(-5/-2)
1047 GCTGAGG
BciVI 49 GGATAC GTATCC(6/5)
334 GGATAC
BcnI 668 CCCGG CC^[GC]GG
669 CCGGG
BpuAI 1084 GAAGAC GAAGAC(2/6)
1204 GTCTTC
BsaI 421 GAGACC GGTCTC(1/5)
964 GAGACC
BseRI 1066 CTCCTC GAGGAG(10/8)
1033 GAGGAG
BsgI 1055 CTGCAC GTGCAG(16/14)
208 GTGCAG
BsiHKAI 26 GAGCTC G[AT]GC[AT]^C
1168 GTGCAC
BsmBI 700 CGTCTC CGTCTC(1/5)
1036 GAGACG
BspHI 830 TCATGA T^CATGA
1080 TCATGA
BstYI 411 GGATCT [GA]^GATC[CT]
1274 AGATCC
DraIII 1337 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1465 CACGATGTG
DrdI 692 GACTCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
966 GACCAAGCAGTC
EcoNI 585 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
945 CCTGGGTGAGG
HgaI 1038 GACGC GACGC(5/10)
699 GCGTC
HinfI 471 GAGTC G^A[ACGT]TC
692 GACTC
KpnI 112 GGTACC GGTAC^C
798 GGTACC
MflI 411 GGATCT [GA]^GATC[CT]
1274 AGATCC
NciI 668 CCCGG CC^[GC]GG
669 CCGGG
PleI 692 GACTC GAGTC(4/5)
471 GAGTC
PpuMI 581 AGGACCT [GA]G^G[AT]CC[CT]
941 AGGTCCT
RcaI 830 TCATGA T^CATGA
1080 TCATGA
SfcI 589 CTACAG C^T[GA][CT]AG
1496 CTACAG
XhoII 411 GGATCT [GA]^GATC[CT]
1274 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeI
MboI MboII MnlI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PflMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI Van91I