Restriction map for
pFN21AE5674
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
BsmI BspDI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MluI MluNI
MscI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1160 GTCTAC GT^[AC][GT]AC
AccIII 405 TCCGGA T^CCGGA
AflII 460 CTTAAG C^TTAAG
AflIII 1419 ACATGT A^C[GA][CT]GT
AlwNI 955 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 816 ATTAAT AT^TAAT
AsnI 816 ATTAAT AT^TAAT
Asp700I 1546 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1651 CCCGAG C^[CT]CG[GA]G
BanII 270 GGGCTC G[GA]GC[CT]^C
BcnI 1623 CCCGG CC^[GC]GG
BfrI 460 CTTAAG C^TTAAG
BglII 546 AGATCT A^GATCT
BmyI 270 GGGCTC G[AGT]GC[ACT]^C
BsaWI 405 TCCGGA [AT]^CCGG[AT]
BseAI 405 TCCGGA T^CCGGA
BsoBI 1651 CCCGAG C^[CT]CG[GA]G
Bsp1286I 270 GGGCTC G[AGT]GC[ACT]^C
BspEI 405 TCCGGA T^CCGGA
BspLU11I 1419 ACATGT A^CATGT
BspMI 1616 ACCTGC ACCTGC(4/8)
BstAPI 580 GCAACTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1627 GCTAGC GC[ACGT]^[ACGT]GC
DsaI 381 CCATGG C^C[GA][CT]GG
EcoNI 1125 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 977 GAATTC G^AATTC
HgaI 120 GCGTC GACGC(5/10)
HincII 838 GTTGAC GT[CT]^[GA]AC
HindII 838 GTTGAC GT[CT]^[GA]AC
HindIII 112 AAGCTT A^AGCTT
MaeII 310 ACGT A^CGT
MroI 405 TCCGGA T^CCGGA
MspA1I 177 CAGCTG C[AC]G^C[GT]G
NciI 1623 CCCGG CC^[GC]GG
NcoI 381 CCATGG C^CATGG
NheI 1627 GCTAGC G^CTAGC
NspI 1419 ACATGT [GA]CATG^[CT]
PflMI 637 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1049 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 816 ATTAAT AT^TAAT
PvuII 177 CAGCTG CAG^CTG
SapI 1096 GCTCTTC GCTCTTC(1/4)
SbfI 346 CCTGCAGG CCTGCA^GG
ScaI 668 AGTACT AGT^ACT
SmlI 460 CTTAAG C^T[CT][GA]AG
Sse8387I 346 CCTGCAGG CCTGCA^GG
TaqI 744 TCGA T^CGA
TthHB8I 744 TCGA T^CGA
Van91I 637 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 940 TCTAGA T^CTAGA
XmnI 1546 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbsI 507 GAAGAC GAAGAC(2/6)
1597 GAAGAC
BclI 1025 TGATCA T^GATCA
1229 TGATCA
BfaI 941 CTAG C^TAG
1628 CTAG
BpmI 96 CTGGAG CTGGAG(16/14)
155 CTGGAG
BpuAI 507 GAAGAC GAAGAC(2/6)
1597 GAAGAC
Bsp1407I 414 TGTACA T^GTACA
794 TGTACA
BsrGI 414 TGTACA T^GTACA
794 TGTACA
DraII 464 AGGGCCT [GA]G^G[ACGT]CC[CT]
1049 AGGACCC
DraIII 1237 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1366 CACCCTGTG
EaeI 1152 TGGCCG [CT]^GGCC[GA]
1203 TGGCCG
EcoO109I 464 AGGGCCT [GA]G^G[ACGT]CC[CT]
1049 AGGACCC
FbaI 1025 TGATCA T^GATCA
1229 TGATCA
HapII 406 CCGG C^CGG
1624 CCGG
HpaII 406 CCGG C^CGG
1624 CCGG
MaeI 941 CTAG C^TAG
1628 CTAG
MfeI 773 CAATTG C^AATTG
806 CAATTG
MspI 406 CCGG C^CGG
1624 CCGG
MunI 773 CAATTG C^AATTG
806 CAATTG
MwoI 411 GCTTGTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
580 GCAACTAATGC
PstI 347 CTGCAG CTGCA^G
961 CTGCAG
SspBI 414 TGTACA T^GTACA
794 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII