Restriction map for
pFN21AE5669
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsiWI BsmBI BsmI Bsp1407I BspHI
BspLU11I BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I CelII CpoI
DraI DraIII DrdI Eam1105I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfoI
SfuI SgrAI SmlI SpeI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 418 TCCGGA T^CCGGA
AocI 1572 CCTGAGG CC^T[ACGT]AGG
Aor51HI 707 AGCGCT AGC^GCT
Asp700I 1242 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1567 GAGCAC G[AT]GC[AT]^C
AspI 667 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 289 GGGCTC G[GA]GC[CT]^C
BbsI 1024 GAAGAC GAAGAC(2/6)
BfaI 448 CTAG C^TAG
BglII 476 AGATCT A^GATCT
BpmI 1156 CTCCAG CTGGAG(16/14)
BpuAI 1024 GAAGAC GAAGAC(2/6)
BsaI 337 GAGACC GGTCTC(1/5)
BsaWI 418 TCCGGA [AT]^CCGG[AT]
BseAI 418 TCCGGA T^CCGGA
BsiEI 275 CGGCCG CG[GA][CT]^CG
BsiHKAI 1567 GAGCAC G[AT]GC[AT]^C
BspDI 403 ATCGAT AT^CGAT
BspEI 418 TCCGGA T^CCGGA
BsrDI 684 CATTGC GCAATG(2/0)
BsrFI 218 GCCGGC [GA]^CCGG[CT]
BstEII 579 GGTCACC G^GT[ACGT]ACC
BstPI 579 GGTCACC G^GT[ACGT]ACC
BstXI 1231 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1572 CCTGAGG CC^T[ACGT]AGG
Cfr10I 218 GCCGGC [GA]^CCGG[CT]
ClaI 403 ATCGAT AT^CGAT
DraII 1234 AGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 275 CGGCCG C^GGCCG
EclXI 275 CGGCCG C^GGCCG
Eco47III 707 AGCGCT AGC^GCT
Eco52I 275 CGGCCG C^GGCCG
Eco81I 1572 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1234 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 579 GGTCACC G^GT[ACGT]ACC
HaeII 707 AGCGCT [GA]GCGC^[CT]
MaeI 448 CTAG C^TAG
MroI 418 TCCGGA T^CCGGA
MseI 1298 TTAA T^TAA
MslI 870 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 218 GCCGGC GCC^GGC
NgoMIV 218 GCCGGC G^CCGGC
NspI 208 GCATGC [GA]CATG^[CT]
PflFI 667 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 626 AGTACT AGT^ACT
SfiI 276 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 488 CCCGGG CCC^GGG
SnaBI 1521 TACGTA TAC^GTA
SphI 208 GCATGC GCATG^C
TfiI 1587 GAATC G^A[AT]TC
Tru9I 1298 TTAA T^TAA
Tth111I 667 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 488 CCCGGG C^CCGGG
XmaI 488 CCCGGG C^CCGGG
XmnI 1242 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 969 GATCC GGATC(4/5)
401 GGATC
AvaI 488 CCCGGG C^[CT]CG[GA]G
1335 CCCGAG
AvaII 444 GGACC G^G[AT]CC
666 GGACC
BalI 701 TGGCCA TGG^CCA
803 TGGCCA
BglI 277 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1154 GCCTCCAGGGC
BsaAI 619 TACGTG [CT]AC^GT[GA]
1521 TACGTA
BseRI 760 CTCCTC GAGGAG(10/8)
1056 CTCCTC
BsoBI 488 CCCGGG C^[CT]CG[GA]G
1335 CCCGAG
BspMI 500 ACCTGC ACCTGC(4/8)
1028 ACCTGC
BssHII 10 GCGCGC G^CGCGC
948 GCGCGC
BstYI 476 AGATCT [GA]^GATC[CT]
968 AGATCC
EarI 1006 CTCTTC CTCTTC(1/4)
1180 CTCTTC
HgaI 1126 GACGC GACGC(5/10)
56 GCGTC
Ksp632I 1006 CTCTTC CTCTTC(1/4)
1180 CTCTTC
MflI 476 AGATCT [GA]^GATC[CT]
968 AGATCC
MluNI 701 TGGCCA TGG^CCA
803 TGGCCA
MscI 701 TGGCCA TGG^CCA
803 TGGCCA
NcoI 584 CCATGG C^CATGG
1088 CCATGG
NlaIV 825 GGCTCC GG[ACGT]^[ACGT]CC
1469 GGAACC
PvuII 358 CAGCTG CAG^CTG
1078 CAGCTG
SexAI 197 ACCTGGT A^CC[AT]GGT
668 ACCTGGT
TaqI 404 TCGA T^CGA
730 TCGA
TthHB8I 404 TCGA T^CGA
730 TCGA
XcmI 387 CCACACCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
713 CCAAGTGTCTCCTGG
XhoII 476 AGATCT [GA]^GATC[CT]
968 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
BbvI BcnI BmyI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI