Restriction map for
pFN21AE5646
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvI
BcgI BcnI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraI DraII DraIII
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI HinP1I HpaI HpaII KasI
KpnI KspI MfeI MluI MroI
MslI MspI MunI MvaI MwoI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI TfiI TseI
Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 31 GACGTC GACGT^C
AccII 65 CGCG CG^CG
AcyI 31 GACGTC G[GA]^CG[CT]C
AflIII 933 ACATGT A^C[GA][CT]GT
AlwNI 170 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 885 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 31 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1337 CTCGAG C^[CT]CG[GA]G
BbvCI 613 GCTGAGG CCTCAGC(-5/-2)
BciVI 1077 GGATAC GTATCC(6/5)
BglII 1389 AGATCT A^GATCT
BsaBI 757 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 31 GACGTC G[GA]^CG[CT]C
BseRI 185 GAGGAG GAGGAG(10/8)
BsiEI 61 CGGCCG CG[GA][CT]^CG
BsiYI 53 CCGAAGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 53 CCGAAGAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 889 GCATTC GAATGC(1/-1)
BsoBI 1337 CTCGAG C^[CT]CG[GA]G
BspDI 527 ATCGAT AT^CGAT
BspLU11I 933 ACATGT A^CATGT
BstF5I 1347 GGATG GGATG(2/0)
BstUI 65 CGCG CG^CG
BstXI 204 CCAACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 790 GGGCC G^G[ACGT]CC
ClaI 527 ATCGAT AT^CGAT
DrdI 828 GACAGGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 61 CGGCCG C^GGCCG
EarI 1180 GAAGAG CTCTTC(1/4)
EclXI 61 CGGCCG C^GGCCG
Eco52I 61 CGGCCG C^GGCCG
EcoT14I 284 CCATGG C^C[AT][AT]GG
Fnu4HI 63 GCCGC GC^[ACGT]GC
FokI 1347 GGATG GGATG(9/13)
Hin1I 31 GACGTC G[GA]^CG[CT]C
HincII 430 GTTGAC GT[CT]^[GA]AC
HindII 430 GTTGAC GT[CT]^[GA]AC
HindIII 225 AAGCTT A^AGCTT
ItaI 63 GCCGC GC^[ACGT]GC
Ksp632I 1180 GAAGAG CTCTTC(1/4)
MaeII 32 ACGT A^CGT
MamI 757 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 611 CAGCTG C[AC]G^C[GT]G
MvnI 65 CGCG CG^CG
NcoI 284 CCATGG C^CATGG
PaeR7I 1337 CTCGAG C^TCGAG
PflFI 31 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 611 CAGCTG CAG^CTG
Sau96I 790 GGGCC G^G[ACGT]CC
SfaNI 694 GATGC GCATC(5/9)
SmlI 1337 CTCGAG C^T[CT][GA]AG
StyI 284 CCATGG C^C[AT][AT]GG
Tth111I 31 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 366 CCATACGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1337 CTCGAG C^TCGAG
XmnI 885 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 64 CCGC CCGC(-3/-1)
4 GCGG
AcsI 78 GAATTT [GA]^AATT[CT]
859 GAATTT
ApoI 78 GAATTT [GA]^AATT[CT]
859 GAATTT
AseI 415 ATTAAT AT^TAAT
516 ATTAAT
AsnI 415 ATTAAT AT^TAAT
516 ATTAAT
BalI 201 TGGCCA TGG^CCA
1350 TGGCCA
BbsI 825 GAAGAC GAAGAC(2/6)
1114 GAAGAC
BclI 216 TGATCA T^GATCA
303 TGATCA
BfaI 346 CTAG C^TAG
782 CTAG
BpmI 710 CTCCAG CTGGAG(16/14)
13 CTGGAG
BpuAI 825 GAAGAC GAAGAC(2/6)
1114 GAAGAC
BsaJI 180 CCGTGG C^C[ACGT][ACGT]GG
284 CCATGG
Bsp1407I 47 TGTACA T^GTACA
132 TGTACA
BsrGI 47 TGTACA T^GTACA
132 TGTACA
DsaI 180 CCGTGG C^C[GA][CT]GG
284 CCATGG
EcoRV 457 GATATC GAT^ATC
1228 GATATC
FbaI 216 TGATCA T^GATCA
303 TGATCA
MaeI 346 CTAG C^TAG
782 CTAG
MluNI 201 TGGCCA TGG^CCA
1350 TGGCCA
MscI 201 TGGCCA TGG^CCA
1350 TGGCCA
NspI 933 ACATGT [GA]CATG^[CT]
1202 ACATGC
PshBI 415 ATTAAT AT^TAAT
516 ATTAAT
SfcI 167 CTACAG C^T[GA][CT]AG
1233 CTATAG
SspBI 47 TGTACA T^GTACA
132 TGTACA
SspI 104 AATATT AAT^ATT
774 AATATT
XbaI 345 TCTAGA T^CTAGA
781 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI AlwI BsaI BsmAI
BsmFI BsrI BstYI Cac8I DdeI
DpnII EaeI HaeIII HinfI HphI
MaeIII MboI MboII MflI MnlI
MseI NdeII NlaIII PleI RsaI
Sau3AI TaqI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoII