Restriction map for
pFN21AE5638
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvaI AviII BanI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HapII HpaII KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 616 GCGG CCGC(-3/-1)
AcyI 1092 GACGCC G[GA]^CG[CT]C
AflIII 312 ACGTGT A^C[GA][CT]GT
Alw44I 193 GTGCAC G^TGCAC
AlwNI 55 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 995 GGGCCC GGGCC^C
ApaLI 193 GTGCAC G^TGCAC
Asp700I 572 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 193 GTGCAC G[AT]GC[AT]^C
AspI 532 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1566 CCTAGG C^CTAGG
BalI 668 TGGCCA TGG^CCA
BamHI 1441 GGATCC G^GATCC
BciVI 181 GTATCC GTATCC(6/5)
BglI 997 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1566 CCTAGG C^CTAGG
BsaHI 1092 GACGCC G[GA]^CG[CT]C
BsgI 551 CTGCAC GTGCAG(16/14)
BsiHKAI 193 GTGCAC G[AT]GC[AT]^C
BsmFI 1083 GTCCC GGGAC(10/14)
Bsp1407I 140 TGTACA T^GTACA
BspHI 1217 TCATGA T^CATGA
BspMI 266 ACCTGC ACCTGC(4/8)
BsrGI 140 TGTACA T^GTACA
BstAPI 42 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 449 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 668 TGGCCA [CT]^GGCC[GA]
EcoRV 1291 GATATC GAT^ATC
HaeII 411 GGCGCT [GA]GCGC^[CT]
HgaI 1092 GACGC GACGC(5/10)
Hin1I 1092 GACGCC G[GA]^CG[CT]C
HindIII 303 AAGCTT A^AGCTT
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MslI 576 CACTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 20 CCATGG C^CATGG
PflFI 532 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1081 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1217 TCATGA T^CATGA
SapI 787 GAAGAGC GCTCTTC(1/4)
SfcI 162 CTGTAG C^T[GA][CT]AG
SspBI 140 TGTACA T^GTACA
SspI 1327 AATATT AAT^ATT
StuI 105 AGGCCT AGG^CCT
Tth111I 532 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 904 TCTAGA T^CTAGA
XmnI 572 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 168 AAATTT [GA]^AATT[CT]
1495 GAATTT
AfaI 141 GTAC GT^AC
1100 GTAC
ApoI 168 AAATTT [GA]^AATT[CT]
1495 GAATTT
AvaII 1082 GGTCC G^G[AT]CC
1227 GGTCC
BpmI 767 CTCCAG CTGGAG(16/14)
214 CTGGAG
BseRI 646 CTCCTC GAGGAG(10/8)
1039 GAGGAG
BsmI 612 GAATGC GAATGC(1/-1)
1457 GCATTC
BstYI 503 AGATCC [GA]^GATC[CT]
1441 GGATCC
CfoI 412 GCGC GCG^C
457 GCGC
DraII 994 AGGGCCC [GA]G^G[ACGT]CC[CT]
1081 AGGTCCC
DsaI 20 CCATGG C^C[GA][CT]GG
980 CCACGG
EcoO109I 994 AGGGCCC [GA]G^G[ACGT]CC[CT]
1081 AGGTCCC
HhaI 412 GCGC GCG^C
457 GCGC
HinP1I 412 GCGC G^CGC
457 GCGC
HpaI 597 GTTAAC GTT^AAC
1482 GTTAAC
MaeII 312 ACGT A^CGT
1548 ACGT
MflI 503 AGATCC [GA]^GATC[CT]
1441 GGATCC
PflMI 54 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCAAGTGGTGG
RsaI 141 GTAC GT^AC
1100 GTAC
SmlI 713 CTCAAG C^T[CT][GA]AG
1618 CTCAAG
TfiI 148 GAATC G^A[AT]TC
914 GAATC
Van91I 54 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1161 CCAAGTGGTGG
XhoII 503 AGATCC [GA]^GATC[CT]
1441 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AluI AlwI BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I