Restriction map for
pFN21AB7877
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaBI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 302 GGTACC G^GTACC
AccIII 212 TCCGGA T^CCGGA
AcyI 219 GGCGCC G[GA]^CG[CT]C
AflIII 1393 ACATGT A^C[GA][CT]GT
Asp718I 302 GGTACC G^GTACC
AvaI 237 CCCGGG C^[CT]CG[GA]G
BalI 128 TGGCCA TGG^CCA
BamHI 1343 GGATCC G^GATCC
BbeI 219 GGCGCC GGCGC^C
BbvCI 489 GCTGAGG CCTCAGC(-5/-2)
BfaI 1015 CTAG C^TAG
BglI 1164 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 231 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 231 GCTGAGC GC^T[ACGT]AGC
BsaHI 219 GGCGCC G[GA]^CG[CT]C
BsaWI 212 TCCGGA [AT]^CCGG[AT]
BseAI 212 TCCGGA T^CCGGA
BseRI 595 GAGGAG GAGGAG(10/8)
BsoBI 237 CCCGGG C^[CT]CG[GA]G
BspEI 212 TCCGGA T^CCGGA
BspLU11I 1393 ACATGT A^CATGT
BspMI 602 ACCTGC ACCTGC(4/8)
BssSI 355 CACGAG CACGAG(-5/-1)
BstXI 393 CCAAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 231 GCTGAGC GC^T[ACGT]AGC
CfoI 220 GCGC GCG^C
DrdI 1597 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1001 CCAAGG C^C[AT][AT]GG
HaeII 219 GGCGCC [GA]GCGC^[CT]
HgaI 178 GCGTC GACGC(5/10)
HhaI 220 GCGC GCG^C
Hin1I 219 GGCGCC G[GA]^CG[CT]C
HinP1I 220 GCGC G^CGC
HincII 982 GTCAAC GT[CT]^[GA]AC
HindII 982 GTCAAC GT[CT]^[GA]AC
KasI 219 GGCGCC G^GCGCC
KpnI 302 GGTACC GGTAC^C
MaeI 1015 CTAG C^TAG
MaeII 317 ACGT A^CGT
MluNI 128 TGGCCA TGG^CCA
MroI 212 TCCGGA T^CCGGA
MscI 128 TGGCCA TGG^CCA
MseI 445 TTAA T^TAA
MspA1I 163 CAGCTG C[AC]G^C[GT]G
NarI 219 GGCGCC GG^CGCC
PflMI 790 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 163 CAGCTG CAG^CTG
SacI 1081 GAGCTC GAGCT^C
ScaI 1559 AGTACT AGT^ACT
SfoI 219 GGCGCC GGC^GCC
SmaI 237 CCCGGG CCC^GGG
SmlI 1255 CTCAAG C^T[CT][GA]AG
SspI 1235 AATATT AAT^ATT
StuI 672 AGGCCT AGG^CCT
StyI 1001 CCAAGG C^C[AT][AT]GG
Tru9I 445 TTAA T^TAA
Van91I 790 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 237 CCCGGG C^CCGGG
XmaI 237 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 81 CGCG CG^CG
363 CGCG
AcsI 1114 AAATTT [GA]^AATT[CT]
1291 AAATTT
AocI 31 CCTCAGG CC^T[ACGT]AGG
481 CCTGAGG
ApaI 841 GGGCCC GGGCC^C
1171 GGGCCC
ApoI 1114 AAATTT [GA]^AATT[CT]
1291 AAATTT
AspHI 640 GAGCAC G[AT]GC[AT]^C
1081 GAGCTC
BglII 381 AGATCT A^GATCT
911 AGATCT
BsiHKAI 640 GAGCAC G[AT]GC[AT]^C
1081 GAGCTC
BspHI 1362 TCATGA T^CATGA
1701 TCATGA
BsrFI 1334 ACCGGC [GA]^CCGG[CT]
1373 ACCGGC
BstAPI 175 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1579 GCATCCCATGC
BstUI 81 CGCG CG^CG
363 CGCG
Bsu36I 31 CCTCAGG CC^T[ACGT]AGG
481 CCTGAGG
Cfr10I 1334 ACCGGC [GA]^CCGG[CT]
1373 ACCGGC
DsaI 134 CCGTGG C^C[GA][CT]GG
298 CCGTGG
EarI 710 CTCTTC CTCTTC(1/4)
1180 GAAGAG
Eco81I 31 CCTCAGG CC^T[ACGT]AGG
481 CCTGAGG
HphI 781 GGTGA GGTGA(8/7)
517 TCACC
Ksp632I 710 CTCTTC CTCTTC(1/4)
1180 GAAGAG
MvnI 81 CGCG CG^CG
363 CGCG
NspI 1393 ACATGT [GA]CATG^[CT]
1436 ACATGC
PpuMI 493 AGGACCT [GA]G^G[AT]CC[CT]
599 AGGACCT
PstI 807 CTGCAG CTGCA^G
1187 CTGCAG
RcaI 1362 TCATGA T^CATGA
1701 TCATGA
TfiI 706 GATTC G^A[AT]TC
1065 GATTC
Tsp45I 625 GTCAC ^GT[GC]AC
1144 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp509I
TspRI XcmI XhoII XmnI