##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MroI        MseI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI          125  GCGG                 CCGC(-3/-1)
AfaI           49  GTAC                 GT^AC
Aor51HI         5  AGCGCT               AGC^GCT
Asp700I         3  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          149  TGGCCA               TGG^CCA
BbvCI         104  CCTCAGC              CCTCAGC(-5/-2)
BbvI           98  GCAGC                GCAGC(8/12)
BsiYI          39  CCTGGTTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           39  CCTGGTTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrI           61  ACTGG                ACTGG(1/-1)
BssKI          39  CCTGG                ^CC[ACGT]GG
BstF5I        110  CATCC                GGATG(2/0)
BstNI          39  CCTGG                CC^[AT]GG
CfoI            6  GCGC                 GCG^C
EaeI          149  TGGCCA               [CT]^GGCC[GA]
Eco47III        5  AGCGCT               AGC^GCT
EcoRII         39  CCTGG                ^CC[AT]GG
Fnu4HI         98  GCAGC                GC^[ACGT]GC
FokI          110  CATCC                GGATG(9/13)
HaeII           5  AGCGCT               [GA]GCGC^[CT]
HaeIII        150  GGCC                 GG^CC
HhaI            6  GCGC                 GCG^C
HinP1I          6  GCGC                 G^CGC
HinfI          65  GACTC                G^A[ACGT]TC
HphI           26  TCACC                GGTGA(8/7)
ItaI           98  GCAGC                GC^[ACGT]GC
MluNI         149  TGGCCA               TGG^CCA
MscI          149  TGGCCA               TGG^CCA
MvaI           39  CCTGG                CC^[AT]GG
PleI           65  GACTC                GAGTC(4/5)
RsaI           49  GTAC                 GT^AC
ScrFI          39  CCTGG                CC^[ACGT]GG
SfaNI         109  GCATC                GCATC(5/9)
TseI           98  GCAGC                G^C[AT]GC
XcmI           29  CCATCAGCCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI            3  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

DdeI           69  CTCAG                C^T[ACGT]AG
              105  CTCAG               
EarI           13  CTCTTC               CTCTTC(1/4)
              169  CTCTTC              
Ksp632I        13  CTCTTC               CTCTTC(1/4)
              169  CTCTTC              
MwoI           89  GCCAAACCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              116  GCAACATAAGC         


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MboII       MnlI