Restriction map for
pFN21AB8954
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspHI AspI AvaII
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DraII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I KspI MamI MfeI MflI
MluNI MroI MscI MseI MunI
NcoI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 487 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 587 GGATC GGATC(4/5)
AocI 262 CCTCAGG CC^T[ACGT]AGG
AscI 303 GGCGCGCC GG^CGCGCC
AspEI 487 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 5 CGACTTCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 292 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 808 GTGCAG GTGCAG(16/14)
BsmAI 723 GTCTC GTCTC(1/5)
BsmFI 668 GGGAC GGGAC(10/14)
BssHII 304 GCGCGC G^CGCGC
BstEII 909 GGTAACC G^GT[ACGT]ACC
BstPI 909 GGTAACC G^GT[ACGT]ACC
Bsu36I 262 CCTCAGG CC^T[ACGT]AGG
DpnII 588 GATC ^GATC
Eam1105I 487 GACGACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 262 CCTCAGG CC^T[ACGT]AGG
EcoO65I 909 GGTAACC G^GT[ACGT]ACC
MboI 588 GATC ^GATC
MboII 945 GAAGA GAAGA(8/7)
MluI 789 ACGCGT A^CGCGT
MslI 801 CACTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 588 GATC ^GATC
NheI 841 GCTAGC G^CTAGC
NlaIII 168 CATG CATG^
PflMI 659 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI 588 GATC ^GATC
SgrAI 868 CGCCGGCG C[GA]^CCGG[CT]G
Van91I 659 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 344 CCAGCGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 48 GTAC GT^AC
681 GTAC
AflIII 569 ACGTGT A^C[GA][CT]GT
789 ACGCGT
AlwNI 183 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
198 CAGCCACTG
BanII 147 GAGCCC G[GA]GC[CT]^C
650 GGGCTC
BseRI 98 GAGGAG GAGGAG(10/8)
101 GAGGAG
BspMI 212 ACCTGC ACCTGC(4/8)
416 ACCTGC
BsrBI 75 GAGCGG CCGCTC(-3/-3)
122 GAGCGG
BsrI 647 ACTGG ACTGG(1/-1)
309 CCAGT
BstAPI 182 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
222 GCACCCCGTGC
DraIII 223 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
406 CACAACGTG
EaeI 31 TGGCCG [CT]^GGCC[GA]
194 CGGCCA
EcoT14I 506 CCAAGG C^C[AT][AT]GG
689 CCAAGG
HgaI 616 GACGC GACGC(5/10)
609 GCGTC
NaeI 243 GCCGGC GCC^GGC
869 GCCGGC
NgoMIV 243 GCCGGC G^CCGGC
869 GCCGGC
PvuII 186 CAGCTG CAG^CTG
811 CAGCTG
RsaI 48 GTAC GT^AC
681 GTAC
SmaI 444 CCCGGG CCC^GGG
583 CCCGGG
StyI 506 CCAAGG C^C[AT][AT]GG
689 CCAAGG
TaqI 632 TCGA T^CGA
964 TCGA
Tsp45I 485 GTGAC ^GT[GC]AC
611 GTCAC
TthHB8I 632 TCGA T^CGA
964 TCGA
XmaCI 444 CCCGGG C^CCGGG
583 CCCGGG
XmaI 444 CCCGGG C^CCGGG
583 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BbeI BbvI BcnI BfaI
BmyI BsaHI BsaJI BsiYI BslI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeI MaeII MaeIII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NlaIV PstI Sau96I
ScrFI SfaNI SfcI SfoI TseI
TspRI