Restriction map for
pFN21AE5023
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbrPI BbsI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraIII DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI MaeI MamI MboI MfeI
MflI MluI MroI MseI MunI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SalI Sau3AI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 485 GGCGCC G[GA]^CG[CT]C
AflIII 791 ACGTGT A^C[GA][CT]GT
Alw44I 1021 GTGCAC G^TGCAC
Aor51HI 350 AGCGCT AGC^GCT
ApaLI 1021 GTGCAC G^TGCAC
AspI 1123 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 995 TGGCCA TGG^CCA
BanI 485 GGCGCC G^G[CT][GA]CC
BbeI 485 GGCGCC GGCGC^C
BbvCI 1173 CCTCAGC CCTCAGC(-5/-2)
BlpI 1161 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1161 GCTCAGC GC^T[ACGT]AGC
BsaHI 485 GGCGCC G[GA]^CG[CT]C
BsaI 667 GAGACC GGTCTC(1/5)
BsmAI 667 GAGAC GTCTC(1/5)
BsrBI 622 GAGCGG CCGCTC(-3/-3)
BstAPI 221 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1203 GGTCACC G^GT[ACGT]ACC
BstPI 1203 GGTCACC G^GT[ACGT]ACC
BstXI 833 CCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1161 GCTCAGC GC^T[ACGT]AGC
Eco47III 350 AGCGCT AGC^GCT
EcoNI 1287 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1203 GGTCACC G^GT[ACGT]ACC
Hin1I 485 GGCGCC G[GA]^CG[CT]C
HinfI 1366 GACTC G^A[ACGT]TC
HphI 1205 TCACC GGTGA(8/7)
KasI 485 GGCGCC G^GCGCC
MluNI 995 TGGCCA TGG^CCA
MscI 995 TGGCCA TGG^CCA
NarI 485 GGCGCC GG^CGCC
PflFI 1123 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1366 GACTC GAGTC(4/5)
SacI 1243 GAGCTC GAGCT^C
SfiI 564 GGCCGTCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 485 GGCGCC GGC^GCC
SmlI 1246 CTCAAG C^T[CT][GA]AG
TspRI 1016 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1123 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1100 CCATGACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 285 GGGCCC GGGCC^C
1338 GGGCCC
AscI 339 GGCGCGCC GG^CGCGCC
906 GGCGCGCC
BcgI 530 GCAAGCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
650 GCAGCCTGTTCG
BsaAI 790 TACGTG [CT]AC^GT[GA]
892 TACGTG
BspMI 164 ACCTGC ACCTGC(4/8)
1149 GCAGGT
BsrFI 479 GCCGGC [GA]^CCGG[CT]
1281 GCCGGC
BsrI 323 ACTGG ACTGG(1/-1)
1017 ACTGG
Cfr10I 479 GCCGGC [GA]^CCGG[CT]
1281 GCCGGC
EarI 535 CTCTTC CTCTTC(1/4)
1006 CTCTTC
EcoT14I 7 CCATGG C^C[AT][AT]GG
998 CCATGG
Ksp632I 535 CTCTTC CTCTTC(1/4)
1006 CTCTTC
KspI 150 CCGCGG CCGC^GG
1370 CCGCGG
NaeI 479 GCCGGC GCC^GGC
1281 GCCGGC
NcoI 7 CCATGG C^CATGG
998 CCATGG
NgoMIV 479 GCCGGC G^CCGGC
1281 GCCGGC
PflMI 912 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCAGCTATTGG
PpuMI 40 GGGACCC [GA]G^G[AT]CC[CT]
453 GGGACCT
SacII 150 CCGCGG CCGC^GG
1370 CCGCGG
SapI 534 GCTCTTC GCTCTTC(1/4)
1005 GCTCTTC
SbfI 714 CCTGCAGG CCTGCA^GG
1287 CCTGCAGG
Sse8387I 714 CCTGCAGG CCTGCA^GG
1287 CCTGCAGG
StyI 7 CCATGG C^C[AT][AT]GG
998 CCATGG
Van91I 912 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCAGCTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PstI Sau96I ScrFI SfaNI
SfcI SmaI TaqI TseI Tsp45I
TthHB8I XmaCI XmaI