Restriction map for
pFN21AE5010
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvaII
BamHI BanI BanII BbeI BbrPI
BcgI BclI BcnI BfrI BglI
BglII BlpI BmyI Bpu1102I BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MslI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NlaIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 485 GTATAC GT^[AC][GT]AC
AcsI 98 GAATTT [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
ApoI 98 GAATTT [GA]^AATT[CT]
AspI 538 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 677 TGCGCA TGC^GCA
AvrII 412 CCTAGG C^CTAGG
BbsI 442 GTCTTC GAAGAC(2/6)
BbvCI 126 CCTCAGC CCTCAGC(-5/-2)
BciVI 817 GTATCC GTATCC(6/5)
BlnI 412 CCTAGG C^CTAGG
BpuAI 442 GTCTTC GAAGAC(2/6)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsaI 758 GGTCTC GGTCTC(1/5)
BsiEI 360 CGATCG CG[GA][CT]^CG
BsmAI 759 GTCTC GTCTC(1/5)
BsmI 331 GAATGC GAATGC(1/-1)
Bst1107I 485 GTATAC GTA^TAC
BstYI 268 AGATCC [GA]^GATC[CT]
BstZ17I 485 GTATAC GTA^TAC
CfoI 678 GCGC GCG^C
EcoT22I 288 ATGCAT ATGCA^T
FspI 677 TGCGCA TGC^GCA
HhaI 678 GCGC GCG^C
HinP1I 678 GCGC G^CGC
HincII 158 GTCAAC GT[CT]^[GA]AC
HindII 158 GTCAAC GT[CT]^[GA]AC
MaeII 368 ACGT A^CGT
MflI 268 AGATCC [GA]^GATC[CT]
NdeI 650 CATATG CA^TATG
NsiI 288 ATGCAT ATGCA^T
NspI 338 ACATGC [GA]CATG^[CT]
PflFI 538 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 378 CTGCAG CTGCA^G
PvuI 360 CGATCG CGAT^CG
RsaI 177 GTAC GT^AC
Tth111I 538 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 268 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 23 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
107 CAGTTACTG
BalI 371 TGGCCA TGG^CCA
623 TGGCCA
BfaI 413 CTAG C^TAG
791 CTAG
BpmI 567 CTCCAG CTGGAG(16/14)
935 CTCCAG
BsmFI 408 GTCCC GGGAC(10/14)
544 GTCCC
BspMI 783 ACCTGC ACCTGC(4/8)
698 GCAGGT
BssKI 807 CCAGG ^CC[ACGT]GG
937 CCAGG
BstNI 807 CCAGG CC^[AT]GG
937 CCAGG
Cac8I 342 GCTGGC GC[ACGT]^[ACGT]GC
355 GCCTGC
DsaI 350 CCATGG C^C[GA][CT]GG
474 CCATGG
EaeI 371 TGGCCA [CT]^GGCC[GA]
623 TGGCCA
EarI 79 CTCTTC CTCTTC(1/4)
88 CTCTTC
EcoRII 807 CCAGG ^CC[AT]GG
937 CCAGG
HphI 665 TCACC GGTGA(8/7)
803 TCACC
Ksp632I 79 CTCTTC CTCTTC(1/4)
88 CTCTTC
MaeI 413 CTAG C^TAG
791 CTAG
MluNI 371 TGGCCA TGG^CCA
623 TGGCCA
MscI 371 TGGCCA TGG^CCA
623 TGGCCA
MvaI 807 CCAGG CC^[AT]GG
937 CCAGG
NcoI 350 CCATGG C^CATGG
474 CCATGG
ScrFI 807 CCAGG CC^[ACGT]GG
937 CCAGG
SfcI 378 CTGCAG C^T[GA][CT]AG
720 CTGTAG
Tsp45I 25 GTGAC ^GT[GC]AC
536 GTGAC
Tsp509I 99 AATT ^AATT
141 AATT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
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AluI AlwI BbvI BsaJI BstF5I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI MseI MspA1I MwoI
NdeII NlaIII PvuII Sau3AI SfaNI
StyI TfiI Tru9I TseI TspRI