Restriction map for
pFN21AE5003
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspMI BsrBI
BsrFI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvaI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI ScrFI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 40 GTAGAC GT^[AC][GT]AC
AciI 145 CCGC CCGC(-3/-1)
AflII 766 CTTAAG C^TTAAG
AflIII 937 ACATGT A^C[GA][CT]GT
Aor51HI 266 AGCGCT AGC^GCT
AspHI 1085 GAGCAC G[AT]GC[AT]^C
AvaI 578 CTCGGG C^[CT]CG[GA]G
BalI 442 TGGCCA TGG^CCA
BbsI 571 GAAGAC GAAGAC(2/6)
BbvCI 5 CCTCAGC CCTCAGC(-5/-2)
BfrI 766 CTTAAG C^TTAAG
BmyI 1085 GAGCAC G[AGT]GC[ACT]^C
BpuAI 571 GAAGAC GAAGAC(2/6)
BsaI 110 GGTCTC GGTCTC(1/5)
BseRI 20 CTCCTC GAGGAG(10/8)
BsiHKAI 1085 GAGCAC G[AT]GC[AT]^C
BsiYI 1065 CCATTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1065 CCATTTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 111 GTCTC GTCTC(1/5)
BsmFI 587 GGGAC GGGAC(10/14)
BsoBI 578 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1085 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 1220 TGTACA T^GTACA
BspHI 1115 TCATGA T^CATGA
BspLU11I 937 ACATGT A^CATGT
BsrGI 1220 TGTACA T^GTACA
CfoI 267 GCGC GCG^C
EaeI 442 TGGCCA [CT]^GGCC[GA]
Eco47III 266 AGCGCT AGC^GCT
EcoRI 496 GAATTC G^AATTC
EcoT14I 963 CCTTGG C^C[AT][AT]GG
HaeII 266 AGCGCT [GA]GCGC^[CT]
HapII 1201 CCGG C^CGG
HhaI 267 GCGC GCG^C
HinP1I 267 GCGC G^CGC
HincII 132 GTTAAC GT[CT]^[GA]AC
HindII 132 GTTAAC GT[CT]^[GA]AC
HinfI 1197 GAATC G^A[ACGT]TC
HpaI 132 GTTAAC GTT^AAC
HpaII 1201 CCGG C^CGG
HphI 792 GGTGA GGTGA(8/7)
MaeIII 389 GTGAC ^GT[ACGT]AC
MluNI 442 TGGCCA TGG^CCA
MscI 442 TGGCCA TGG^CCA
MslI 700 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1201 CCGG C^CGG
NspI 937 ACATGT [GA]CATG^[CT]
PvuII 847 CAGCTG CAG^CTG
RcaI 1115 TCATGA T^CATGA
SfaNI 436 GCATC GCATC(5/9)
SspBI 1220 TGTACA T^GTACA
StyI 963 CCTTGG C^C[AT][AT]GG
TfiI 1197 GAATC G^A[AT]TC
Tsp45I 389 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 865 GTAC GT^AC
1221 GTAC
BbvI 174 GCAGC GCAGC(8/12)
147 GCTGC
BfaI 386 CTAG C^TAG
1058 CTAG
BsaJI 577 CCTCGG C^C[ACGT][ACGT]GG
963 CCTTGG
BsrDI 510 CATTGC GCAATG(2/0)
395 GCAATG
BstYI 882 AGATCC [GA]^GATC[CT]
1353 GGATCT
Cac8I 119 GCAAGC GC[ACGT]^[ACGT]GC
849 GCTGGC
EarI 835 CTCTTC CTCTTC(1/4)
81 GAAGAG
HaeIII 443 GGCC GG^CC
929 GGCC
Ksp632I 835 CTCTTC CTCTTC(1/4)
81 GAAGAG
MaeI 386 CTAG C^TAG
1058 CTAG
MflI 882 AGATCC [GA]^GATC[CT]
1353 GGATCT
MspA1I 145 CCGCTG C[AC]G^C[GT]G
847 CAGCTG
RsaI 865 GTAC GT^AC
1221 GTAC
TseI 147 GCTGC G^C[AT]GC
174 GCAGC
XhoII 882 AGATCC [GA]^GATC[CT]
1353 GGATCT
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AcsI AluI AlwI AlwNI ApoI
BpmI BsrI BstF5I DdeI DpnII
Fnu4HI FokI ItaI MaeII MboI
MboII MnlI MseI MwoI NdeII
NlaIII Sau3AI SmlI SspI Tru9I
Tsp509I TspRI