Restriction map for
pFN21AE1167
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HindIII
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1229 GACGTC GACGT^C
AcsI 940 AAATTT [GA]^AATT[CT]
AflIII 101 ACGTGT A^C[GA][CT]GT
ApoI 940 AAATTT [GA]^AATT[CT]
Asp700I 1293 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1576 CCCGGG C^[CT]CG[GA]G
BalI 824 TGGCCA TGG^CCA
BciVI 776 GGATAC GTATCC(6/5)
BglI 745 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 1484 GAGGAG GAGGAG(10/8)
BsoBI 1576 CCCGGG C^[CT]CG[GA]G
BspDI 1783 ATCGAT AT^CGAT
BsrFI 717 GCCGGT [GA]^CCGG[CT]
BstXI 1472 CCAAGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 717 GCCGGT [GA]^CCGG[CT]
ClaI 1783 ATCGAT AT^CGAT
DraIII 292 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 821 CCATGG C^C[GA][CT]GG
EagI 1142 CGGCCG C^GGCCG
EclXI 1142 CGGCCG C^GGCCG
Eco52I 1142 CGGCCG C^GGCCG
EcoT14I 821 CCATGG C^C[AT][AT]GG
EcoT22I 872 ATGCAT ATGCA^T
HaeII 1177 AGCGCC [GA]GCGC^[CT]
HpaI 841 GTTAAC GTT^AAC
HphI 1055 TCACC GGTGA(8/7)
MluNI 824 TGGCCA TGG^CCA
MscI 824 TGGCCA TGG^CCA
NcoI 821 CCATGG C^CATGG
NsiI 872 ATGCAT ATGCA^T
PpuMI 379 AGGACCT [GA]G^G[AT]CC[CT]
PstI 815 CTGCAG CTGCA^G
PvuII 493 CAGCTG CAG^CTG
ScaI 1766 AGTACT AGT^ACT
SmaI 1576 CCCGGG CCC^GGG
SmlI 1642 CTTGAG C^T[CT][GA]AG
SpeI 786 ACTAGT A^CTAGT
StyI 821 CCATGG C^C[AT][AT]GG
XmaCI 1576 CCCGGG C^CCGGG
XmaI 1576 CCCGGG C^CCGGG
XmnI 1293 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 332 GACGCC G[GA]^CG[CT]C
1229 GACGTC
AlwI 416 GATCC GGATC(4/5)
585 GATCC
AocI 448 CCTAAGG CC^T[ACGT]AGG
793 CCTCAGG
ApaI 66 GGGCCC GGGCC^C
735 GGGCCC
AspI 661 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1436 GACAGAGTC
BbsI 225 GAAGAC GAAGAC(2/6)
1192 GTCTTC
BlpI 833 GCTTAGC GC^T[ACGT]AGC
1173 GCTCAGC
Bpu1102I 833 GCTTAGC GC^T[ACGT]AGC
1173 GCTCAGC
BpuAI 225 GAAGAC GAAGAC(2/6)
1192 GTCTTC
BsaHI 332 GACGCC G[GA]^CG[CT]C
1229 GACGTC
BsaI 959 GAGACC GGTCTC(1/5)
1557 GGTCTC
BsiEI 719 CGGTCG CG[GA][CT]^CG
1142 CGGCCG
BsmAI 959 GAGAC GTCTC(1/5)
1558 GTCTC
BsmFI 889 GGGAC GGGAC(10/14)
1579 GGGAC
BstYI 415 AGATCC [GA]^GATC[CT]
584 AGATCC
Bsu36I 448 CCTAAGG CC^T[ACGT]AGG
793 CCTCAGG
CelII 833 GCTTAGC GC^T[ACGT]AGC
1173 GCTCAGC
EaeI 824 TGGCCA [CT]^GGCC[GA]
1142 CGGCCG
EarI 90 GAAGAG CTCTTC(1/4)
956 GAAGAG
Eco81I 448 CCTAAGG CC^T[ACGT]AGG
793 CCTCAGG
FauI 175 CCCGC CCCGC(4/6)
158 GCGGG
HgaI 332 GACGC GACGC(5/10)
1190 GCGTC
Hin1I 332 GACGCC G[GA]^CG[CT]C
1229 GACGTC
HincII 199 GTTGAC GT[CT]^[GA]AC
841 GTTAAC
HindII 199 GTTGAC GT[CT]^[GA]AC
841 GTTAAC
Ksp632I 90 GAAGAG CTCTTC(1/4)
956 GAAGAG
MflI 415 AGATCC [GA]^GATC[CT]
584 AGATCC
PflFI 661 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1436 GACAGAGTC
PflMI 1342 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1743 CCAGGGGATGG
PleI 1753 GACTC GAGTC(4/5)
1440 GAGTC
SfcI 815 CTGCAG C^T[GA][CT]AG
1014 CTACAG
TaqI 668 TCGA T^CGA
1784 TCGA
TfiI 478 GAATC G^A[AT]TC
1648 GAATC
Tsp45I 811 GTGAC ^GT[GC]AC
922 GTGAC
Tsp509I 941 AATT ^AATT
1307 AATT
Tth111I 661 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1436 GACAGAGTC
TthHB8I 668 TCGA T^CGA
1784 TCGA
Van91I 1342 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1743 CCAGGGGATGG
XhoII 415 AGATCC [GA]^GATC[CT]
584 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI TspRI