Restriction map for
pFN21AE3317
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AflIII AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AvaI AvrII BbrPI
BciVI BglI BglII BlnI BsaAI
BsaI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I FseI HindIII HpaI
KpnI MluI MroI NaeI NdeI
NgoMIV NheI NotI NruI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PvuI PvuII
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tsp45I Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AclI 1932 AACGTT AA^CGTT
AcyI 130 GGCGCC G[GA]^CG[CT]C
AfaI 1882 GTAC GT^AC
Alw44I 1483 GTGCAC G^TGCAC
AlwNI 2608 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1483 GTGCAC G^TGCAC
Asp700I 1071 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1042 TGCGCA TGC^GCA
BamHI 3 GGATCC G^GATCC
BanI 130 GGCGCC G^G[CT][GA]CC
BbeI 130 GGCGCC GGCGC^C
BbsI 1612 GAAGAC GAAGAC(2/6)
BbvCI 1853 CCTCAGC CCTCAGC(-5/-2)
BbvI 2529 GCAGC GCAGC(8/12)
BcgI 1869 CGAGAACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 206 TGATCA T^GATCA
BcnI 126 CCGGG CC^[GC]GG
BlpI 2605 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2605 GCTCAGC GC^T[ACGT]AGC
BpuAI 1612 GAAGAC GAAGAC(2/6)
BsaBI 564 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 130 GGCGCC G[GA]^CG[CT]C
BsaWI 474 ACCGGA [AT]^CCGG[AT]
BsmBI 1066 GAGACG CGTCTC(1/5)
Bsp1407I 1881 TGTACA T^GTACA
BsrDI 2038 GCAATG GCAATG(2/0)
BsrGI 1881 TGTACA T^GTACA
BstAPI 1045 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1964 TTCGAA TT^CGAA
Cac8I 888 GCTGGC GC[ACGT]^[ACGT]GC
CelII 2605 GCTCAGC GC^T[ACGT]AGC
DraI 1326 TTTAAA TTT^AAA
EcoNI 31 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1988 ATGCAT ATGCA^T
FauI 13 GCGGG CCCGC(4/6)
FbaI 206 TGATCA T^GATCA
FspI 1042 TGCGCA TGC^GCA
HaeII 130 GGCGCC [GA]GCGC^[CT]
HgaI 1145 GACGC GACGC(5/10)
Hin1I 130 GGCGCC G[GA]^CG[CT]C
HphI 2398 GGTGA GGTGA(8/7)
KasI 130 GGCGCC G^GCGCC
KspI 11 CCGCGG CCGC^GG
MamI 564 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 668 CAATTG C^AATTG
MspA1I 11 CCGCGG C[AC]G^C[GT]G
MunI 668 CAATTG C^AATTG
NarI 130 GGCGCC GG^CGCC
NciI 126 CCGGG CC^[GC]GG
NsiI 1988 ATGCAT ATGCA^T
NspV 1964 TTCGAA TT^CGAA
Psp1406I 1932 AACGTT AA^CGTT
RsaI 1882 GTAC GT^AC
SacII 11 CCGCGG CCGC^GG
SapI 1303 GAAGAGC GCTCTTC(1/4)
SfoI 130 GGCGCC GGC^GCC
SfuI 1964 TTCGAA TT^CGAA
SspBI 1881 TGTACA T^GTACA
TseI 2529 GCAGC G^C[AT]GC
XbaI 429 TCTAGA T^CTAGA
XmnI 1071 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 316 GTCTAC GT^[AC][GT]AC
2275 GTAGAC
AccII 12 CGCG CG^CG
42 CGCG
AflII 1471 CTTAAG C^TTAAG
1644 CTTAAG
ApaI 1002 GGGCCC GGGCC^C
2258 GGGCCC
AseI 556 ATTAAT AT^TAAT
1984 ATTAAT
AsnI 556 ATTAAT AT^TAAT
1984 ATTAAT
AspHI 1306 GAGCAC G[AT]GC[AT]^C
1483 GTGCAC
BalI 890 TGGCCA TGG^CCA
2389 TGGCCA
BanII 1002 GGGCCC G[GA]GC[CT]^C
2258 GGGCCC
BfrI 1471 CTTAAG C^TTAAG
1644 CTTAAG
BsgI 1462 GTGCAG GTGCAG(16/14)
2429 GTGCAG
BsiHKAI 1306 GAGCAC G[AT]GC[AT]^C
1483 GTGCAC
BsmAI 1066 GAGAC GTCTC(1/5)
357 GTCTC
BstUI 12 CGCG CG^CG
42 CGCG
EaeI 890 TGGCCA [CT]^GGCC[GA]
2389 TGGCCA
HapII 126 CCGG C^CGG
475 CCGG
HincII 1348 GTTGAC GT[CT]^[GA]AC
1493 GTCAAC
HindII 1348 GTTGAC GT[CT]^[GA]AC
1493 GTCAAC
HpaII 126 CCGG C^CGG
475 CCGG
MaeII 1933 ACGT A^CGT
2490 ACGT
MluNI 890 TGGCCA TGG^CCA
2389 TGGCCA
MscI 890 TGGCCA TGG^CCA
2389 TGGCCA
MspI 126 CCGG C^CGG
475 CCGG
MvnI 12 CGCG CG^CG
42 CGCG
NcoI 2075 CCATGG C^CATGG
2262 CCATGG
PpuMI 1466 AGGACCT [GA]G^G[AT]CC[CT]
1908 AGGTCCT
PshAI 1240 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1351 GACTCCAGTC
PshBI 556 ATTAAT AT^TAAT
1984 ATTAAT
PstI 298 CTGCAG CTGCA^G
2527 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRI
EcoRII EcoRV EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I XhoII